| 一种体内研究病原体致病机理的新方法——信号标签诱变技术 |
| |
| 引用本文： | 姚潇,黄留玉,杨伯伦,苏国富.一种体内研究病原体致病机理的新方法——信号标签诱变技术[J].遗传,2002,24(6):721-726. |
| |
| 作者姓名： | 姚潇 黄留玉 杨伯伦 苏国富 |
| |
| 作者单位： | 1.军事医学科学院生物工程研究所，北京，100071；
2.西安交通大学环境与化学工程学院，西安，710049 |
| |
| 基金项目： | 首都二四八重大创新工程(H010210360119)，国家重点基础研究发展规划(973项目)资助(G1999054103) |
| |
| 摘 要： | 信号标签诱变技术是以整个基因组为基础的研究病原体致病机制，可在体内对毒力基因进行高通量筛选的一种新方法。近几年应用该技术已对十多种病原微生物进行了筛选。这些筛选中除了找到已知的毒力基因外，还都鉴定到了未知的毒力因子。本文就该技术的原理、优缺点、应用的必要条件、技术的改进及应用该技术鉴定到的毒力基因等作一综述。
A Novel Approach to Study Pathogenesis of Pathogens in vivo——Signature-tagged Mutagenesis
YAO Xiao1,2,HUANG Liu-yu1,YANG Bo-lun2,SU Guo-fu1
1.Beijing Institute of Biotechnoloy,Beijing 100071,China;
2.College of Environmental and Chemical Engineering,Xi'an Jiaotong University,Xi'an 710049,China
Abstract:Signature-tagged mutagenesis (STM) is a novel approach to study pathogenesis of pathogens and to screen virulence genes with high throughput in vivo,which is based on whole genome of pathogen in question.In resent years,more than ten species of microbial pathogens have been screened with this technology.There are also unknown virulence factors being identified with exception of known virulence genes identified in all these screens.This article reviews the principle,advantages and current limitations,the requirements,modifications of STM,and to date virulence genes identified by this technology.
Key words：signature-tagged mutagenesis;virulence genes;pathogens;in vivo
|
| 关 键 词： | 信号标签诱变 毒力基因 病原体 体内 |
| 文章编号： | 0253-9772(2002)06-0721-06 |
| 修稿时间： | 2002年4月22日 |
A Novel Approach to Study Pathogenesis of Pathogens in vivo--Signature-tagged Mutagenesis |
| |
| Xiao Yao,Liu-yu Huang,Bo-lun Yang,Guo-fu Su.A Novel Approach to Study Pathogenesis of Pathogens in vivo--Signature-tagged Mutagenesis[J].Hereditas,2002,24(6):721-726. |
| |
| Authors: | Xiao Yao Liu-yu Huang Bo-lun Yang Guo-fu Su |
| |
| Institution: | Beijing Institute of Biotechnoloy, Beijing 100071, China. yaoxiao50@hotmail.com |
| |
| Abstract: | Signature-tagged mutagenesis (STM) is a novel approach to study pathogenesis of pathogens and to screen virulence genes with high throughput in vivo,which is based on whole genome of pathogen in question.In recent years, more than ten species of microbial pathogens have been screened with this technology. There are also unknown virulence factors being identified with exception of known virulence genes identified in all these screens. This article reviews the principle, advantages and current limitations,the requirements, modifications of STM, and to date virulence genes identified by this technology. |
| |
| Keywords: | |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《遗传》浏览原始摘要信息 |
| 点击此处可从《遗传》下载免费的PDF全文 |
|
