水稻苗期低温失绿的遗传分析及基因定位

在早季低温条件下, 籼稻品种Dular的幼苗表现出白化失绿, 而粳稻品种Lemont幼苗表现正常绿色。以Lemont和Dular作亲本构建一个F2群体，通过该群体在早季低温条件下性状的表现，发现Lemont和Dular苗期耐冷性的差异受单个主基因控制，低温下白化失绿等位基因为隐性。将该基因暂时命名为cisc(t)。利用该F2群体，采用集团分离分析(BSA)法将cisc(t)定位在9号染色体上。经过对F2群体中100个典型的白化单株的简单序列长度多态性分析，将该基因定位在5.5 cM的区间内，分别与微卫星标记RM257和RM242相距3.9 cM和1.6 cM。

Genetic analysis and gene mapping of cold-induced seedling chlorosis in rice

We found that the seedlings of indica rice cultivar Dular showed chlorosis but the seedlings of japonica rice cultivar Lemont remained green under natural low temperature in early spring. Using an F2 population of Lemont ´ Dular, we found that the difference of cold tolerance at seedling stage between Dular and Lemont is controlled by a single major gene, with the chlorosis allele being recessive. We named the gene cisc(t). With the help of SSR markers, cisc(t) was mapped in a 5.5 cM interval between SSR markers RM257 and RM242 on chromosome 9.