| 哺乳动物中基因持续表达的策略 |
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| 引用本文： | 汪宗桂,郑文岭,崔东,马文丽.哺乳动物中基因持续表达的策略[J].遗传,2004,26(5):745-748. |
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| 作者姓名： | 汪宗桂 郑文岭 崔东 马文丽 |
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| 作者单位： | 1. 广州流花桥医院肿瘤分子生物学研究所，广州 510010； 2. 华南理工大学食品与生物工程学院，广州 510640； 3. 第一军医大学分子生物学研究所，广州 510515 1. Institute of Molecular Oncology, Guangzhou Liuhuaqiao Hospital, Guangzhou 510010,China; 2. Food and Bio-engineering Department of South China University of Techology, Guangzhou 510640,China; 3. Institute of Molecular Biology, First Uniform Medical University Guangzhou 510515,China |
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| 基金项目： | 国家自然科学基金，广东省广州市科技攻关项目 |
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| 摘 要： | 人类遗传病的治疗越来越依赖于基因水平上的治疗，但外源基因不能在哺乳动物中持续表达的缺陷严重阻碍了这一手段的快速发展。含有转座子载体的染色体基因组整合，裸露质粒DNA转化到肝脏的压力输送，或是在质粒载体中插入真核基因的顺式作用元件如内含子、3’端非翻译区、EBV序列等，都可以在一定程度上赋于外源基因以长期持续的表达，这些策略对于基因治疗的发展具有重要的意义。
Abstract: Gene therapy holds great promises to a variety of inherited diseases. However, the limitations on extended and consistent foreign gene expression has severely hampered the development of applicable gene therapy approaches. Technologies are reviewed here including transponson integration, biolistic measures that pulse the naked plasmid into living organs, or the integration of eukaryotic cis elements into introns, 3′ untranslated regions, or the integration of the EBV sequences, which could assist in the prolonged gene expression of the introduced foreign genes. These strategies may significantly promote the progresses of gene therapy.
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| 关 键 词： | 基因治疗 持续表达 Prolong expression 质粒载体 Key words Gene therapy |
| 文章编号： | 0253-9772(2004)05-0745-04 |
| 修稿时间： | 2003年5月26日 |
The strategies of gene prolong expression in mammals |
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| WANG Zong-Gui.The strategies of gene prolong expression in mammals[J].Hereditas,2004,26(5):745-748. |
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| Authors: | WANG Zong-Gui |
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| Institution: | Institute of Molecular Oncology, Guangzhou Liuhuaqiao Hospital, Guangzhou 510 010, China. wzgui825@sina.com |
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| Abstract: | Gene therapy holds great promises to a variety of inherited diseases. However, the limitations on extended and consistent foreign gene expression has severely hampered the development of applicable gene therapy approaches. Technologies are reviewed here including transponson integration, biolistic measures that pulse the naked plasmid into living organs, or the integration of eukaryotic cis elements into introns, 3' untranslated regions, or the integration of the EBV sequences, which could assist in the prolonged gene expression of the introduced foreign genes. These strategies may significantly promote the progresses of gene therapy. |
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| Keywords: | Gene therapy Prolong expression Plasmid vector |
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