水稻胞质核糖体蛋白基因OsRPS7的克隆与序列分析

以已公布的黑麦胞质核糖体蛋白基因ScRPS7的cDNA序列为信息探针，在中国华大水稻基因组数据库中搜索与之高度同源的基因组重叠群。采用计算机拼接和RT-PCR方法克隆了水稻胞质核糖体蛋白基因的全长cDNA序列，命名为OsRPS7。该cDNA序列全长919bp，编码192个氨基酸；其与黑麦、拟南芥和芸薹的S7核糖体蛋白的氨基酸一致率分别为88%、72%和72%。对OsRPS7 的基因组结构和基因的功能进行了分析和预测。Abstract：Using the cDNA of rye cytoplasmic ribosomal protein ScRPS7 as a query probe, a highly homologous rice genomic contig was obtained from Huada rice genome database. The full-length cDNA sequence of rice cytoplasmic ribosomal protein S7 was assembled by informatics based on the contig. Furthermore, with the two primers designed according to this assembled cDNA, the full-length cDNA of rice ribosomal protein was cloned by RT-PCR and named as OsRPS7. The cDNA was 919bp in length and contained a complete Open Reading Frame (ORF) of 576bp, encoding a protein of 192 amino acid residues. The deduced amino acids of OsRPS7 showed 88%、72% and 72% identity with those from Secale cereale、Arabidopsis thaliana and Brassica oleracea, respectively. The genome structure of OsRPS7 was analyzed, and its function was predicted in this paper.

Molecular Cloning and Sequence Analyzing of Cytoplasmic Ribosomal Protein Gene OsRPS7 from Rice (Oryza sativa L.)

Using the cDNA of rye cytoplasmic ribosomal protein ScRPS7 as a query probe, a highly homologous rice genomic contig was obtained from Huada rice genome database. The full-length cDNA sequence of rice cytoplasmic ribosomal protein S7 was assembled by informatics based on the contig. Furthermore, with the two primers designed according to this assembled cDNA, the full-length cDNA of rice ribosomal protein was cloned by RT-PCR and named as OsRPS7. The cDNA was 919bp in length and contained a complete open reading frame of 576bp, encoding a protein of 192 amino acid residues. The deduced amino acids of OsRPS7 showed 88%?72% and 72% identity with those from Secale cereale?Arabidopsis thaliana and Brassica oleracea, respectively. The genome structure of OsRPS7 was analyzed, and its function was predicted in this paper.