首页 | 本学科首页   官方微博 | 高级检索  
   检索      

抗菌肽D基因导入番茄及转基因植株的鉴定
引用本文:田长恩,王正询,陈韬,周玉萍,黄自然,黄亚东.抗菌肽D基因导入番茄及转基因植株的鉴定[J].遗传,2000,22(2):86-89.
作者姓名:田长恩  王正询  陈韬  周玉萍  黄自然  黄亚东
作者单位:1.广州师范学院生物学系,广州 510405 2.华南农业大学蚕桑系, 广州 510642 1.Department of Biology, Guangzhou Teachers College, Guangzhou 510405 2.Department of Sericulture, South China Agricultural University, Guangzhou 510642,China
基金项目:广州市重点项目 !(96-2-04-02)资助
摘    要:用花粉管通道法将柞蚕抗菌肽D基因导入番茄,获得了转基因植株。应用PCR技术、DNA斑点杂交以及Southern印迹分析表明,抗菌肽D基因已整合到番茄基因组;而且,位于目的基因下游的胭脂碱合成酶基因有表达。青枯假单孢菌活体接种结合高发病大田种植试验结果显示,部分转基因植株的子一代具有较强的抗青枯病能力。 Abstract:By using pollen-tube pathway,antibacterial peptide D(AP-D) gene developed from Chinese oak silkwarm was transferred into tomato.PCR,dot and Southern blot analysis confirmed that stable integration of AP-D gene into the tomato genome had occurred.The expression of NOP gene which was localized at downstream of AP-D gene was confirmed with nopaline detection.In comparision with non-transgenicplants,some transgenic plant expressed a stronger resistance value to bacterialwilt disease (Pseudomonas solanacearum).

关 键 词:抗菌肽D基因  花粉通道法  antibacterial  peptide-D  gene  青枯病  Key  words  番茄  

Transfer of Antibacterial Peptide D Gene into Tomato and Identification of Transgenic Plants
TIAN Chang-en,WANG Zheng-xun,CHEN Tao,ZHOU Yu-ping,HANG Zi-ran,HANG Ya-dong.Transfer of Antibacterial Peptide D Gene into Tomato and Identification of Transgenic Plants[J].Hereditas,2000,22(2):86-89.
Authors:TIAN Chang-en  WANG Zheng-xun  CHEN Tao  ZHOU Yu-ping  HANG Zi-ran  HANG Ya-dong
Abstract:By using pollen tube pathway,antibacterial peptide D(AP D) gene developed from Chinese oak silkwarm was transferred into tomato.PCR,dot and Southern blot analysis confirmed that stable integration of AP D gene into the tomato genome had occurred.The expression of NOP gene which was localized at downstream of AP D gene was confirmed with nopaline detection.In comparision with non transgenicplants,some transgenic plant expressed a stronger resistance value to bacterialwilt disease (Pseudomonas solanacearum).
Keywords:antibacterial peptide  D gene  pollen  tube pathway  tomato  bacterialwilt
本文献已被 CNKI 维普 万方数据 等数据库收录!
点击此处可从《遗传》浏览原始摘要信息
点击此处可从《遗传》下载免费的PDF全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号