| Bt水稻杂交育种中转基因的遗传分析 |
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| 引用本文： | 王忠华,崔海瑞,舒庆尧,项友斌,吴殿星,高明尉,夏英武,成雄鹰.Bt水稻杂交育种中转基因的遗传分析[J].遗传,2000,22(5):309-312. |
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| 作者姓名： | 王忠华 崔海瑞 舒庆尧 项友斌 吴殿星 高明尉 夏英武 成雄鹰 |
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| 作者单位： | 1.浙江大学原子核农业科学研究所,杭州华家池 310029;
2.加拿大渥太华大学生物化学系，渥太华KIN 6N5，加拿大
1.Institute of Nuclear Agricultural Sciences,Zhejiang University,Huajiachi Hangzhou 310029,China;
2.Department of Biochemistry,University of Ottawa,Ottawa KIN 6N5,Canada |
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| 基金项目： | 浙江省科委“九五”重大项目，洛氏基金资助!项目（RF97001＃556） |
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| 摘 要： | 利用PCR、GUS染色和Western印迹杂交技术检测了Bt水稻杂交后代群体,发现在394株GUS阳性株中,共有392株表达Bt蛋白,协同表达株率达99.49%。由此表明,在杂交后代中报告基因Gus和目的基因crylAb紧密连锁遗传与表达。本试验还发现,在BC1、BC1F2和粳粳交F2群体中转基因呈单基因显性遗传,而在籼粳交F2群体中偏离3:1分离。
Abstract:Improved histochemical staining for GUS activity,PCR and Western blotting were used to detect the population of Bt rice crossed to conventional rice varieties.A total of 392 plants expressing Bt toxin protein were found in 394 GUS positive plants.The result demonstrated that cry1Ab gene closely inherited and expressed with reporter gene gus.Therefore,it is possible to develop GUS-assisted-selection to preliminarily identify the Bt gene and study the inheritance of transgenes in (back)cross breeding.Mendelian segragation of reporter gene Gus was observed in F2,BC1 and BC1F2 progenies.Thus indicated that transgenes inherited as a single dominant gene in the progenies of Bt rice crossed to conventional rice varieties.
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| 关 键 词： | Bt水稻 转基因 Bt rice 育种 Key words 遗传分析 |
Genetic Analysis of Transgenes in the Progenies of Bt Rice Crossed to Conventional Rice Varieties |
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| WANG Zhong-hua,CUI Hai-rui,SHU Qing-yao,XIANG You-bing,WU Dian-xing,GAO Ming-wei,XIA Ying-wu,Xiong-ying CHeng,ILLimar Altosaar.Genetic Analysis of Transgenes in the Progenies of Bt Rice Crossed to Conventional Rice Varieties[J].Hereditas,2000,22(5):309-312. |
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| Authors: | WANG Zhong-hua CUI Hai-rui SHU Qing-yao XIANG You-bing WU Dian-xing GAO Ming-wei XIA Ying-wu Xiong-ying CHeng ILLimar Altosaar |
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| Abstract: | Improved histochemical staining for GUS activity,PCR and Western blotting were used to detect the population of Bt rice crossed to conventional rice varieties A total of 392 plants expressing Bt toxin protein were found in 394 GUS positive plants.The result demonstrated that cry1Ab gene closely inherited and expressed with reporter gene gus.Therefore,it is possible to develop GUS assisted selection to preliminarily identify the Bt gene and study the inheritance of transgenes in (back)cross breeding.Mendelian segragation of reporter gene Gus was observed in F2,BC1 and BC1F2 progenies.Thus indicated that transgenes inherited as a single dominant gene in the progenies of Bt rice crossed to conventional rice varieties. |
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| Keywords: | Bt rice transgenes genetic analysis: breeding |
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