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大麦DNA导入小麦产生抗白粉病变异的遗传研究
引用本文:阎新甫,刘文轩,王胜军,王西成,王锡锋.大麦DNA导入小麦产生抗白粉病变异的遗传研究[J].遗传,1994(1).
作者姓名:阎新甫  刘文轩  王胜军  王西成  王锡锋
作者单位:河南省农业科学院小麦研究所!郑州,450002,河南省农业科学院小麦研究所!郑州,450002,河南省农业科学院小麦研究所!郑州,450002,河南省农业科学院小麦研究所!郑州,450002,河南省农业科学院植物保护研究所!450002
摘    要:本研究将抗白粉病的大麦DNA通过花粉管途径直接导入感病的小麦品种花76中,后代出现13株抗白粉病变异株.其中5株在以后的世代中抗性稳定,另8株则继续分离.第2代分离株系的抗病株形成的第3代株系(或株行)中,抗性有分离的株行与无分离的株行比例为1.9:1,而分离株行内抗病株与不抗病株之比为335:1。抗性稳定株系与感病亲本杂交,F1表现高抗病,再与感病亲本回交,后代抗感病株比例为1:1。自交Fn的比例为2.8:1.说明所获得的抗白粉病性受一对完全显性基因控制,抗病为显性.与已知抗白粉病基因的比较表明,这个抗病基因可能是来自大麦的一个新基因。

关 键 词:大麦  小麦  白粉病抗性  外源DNA导入  基因测定

Inheritance of Powdery Mildew Resistance in Variants Obtained by Introducing Barley DNA into Wheat
Yan Xinfu, Liu Wenxuan, Wang Shengjun, WangXicheng, Wang Xifeng.Inheritance of Powdery Mildew Resistance in Variants Obtained by Introducing Barley DNA into Wheat[J].Hereditas,1994(1).
Authors:Yan Xinfu  Liu Wenxuan  Wang Shengjun  WangXicheng  Wang Xifeng
Abstract:13 Variant plants with immunity and high-resistance to powdery mildew were found in D1 generation from introducing resistant barley DNA into susceptible wheat cultivar, through pollen tube pathway after self pollination. Of the variants, 5 plants for the resistance had been stable and the other 8plants segregated in suecessive generation. The ratio of segregating and stab1e plant-rows was 1.9:1 inD3 plant-rows derived from resistant plants of segregating D2-lines, and the ratio of resistant plants andsusceptible plants was 3.35 :1 among the segregating D3 plant-rows. The F1-plants from crosses between stable resistant variants and susceptible parents were high resistant to powdery mildew. The ratio ofresistant and susceptible plants was 1:1 in progenies of backcross of the F1 and susceptib1e parents, andthis ratlo was 2.8:1 in the F2 gencration from the F1 selfing. Thus it can be scen that the resistance ob-tained is comtrolled by a pair of genes, the resistance is dominant. The results in comparison with knownpowdery mildew rcsistance genes in wheat indicated that the resistance gene obtained would be a new onefrom barley.
Keywords:Wheat  Powdery mildew resistance  Exogenous DNA introduction  Gene identification
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