| 饲料原料中转基因成分的PCR检测 |
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| 引用本文： | 王颖,余道坚,康林,章桂明,金显忠,杨伟东,黄佩卿,吴琼,陈枝楠,储成才,程颖慧.饲料原料中转基因成分的PCR检测[J].遗传,2002,24(3):293-296. |
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| 作者姓名： | 王颖 余道坚 康林 章桂明 金显忠 杨伟东 黄佩卿 吴琼 陈枝楠 储成才 程颖慧 |
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| 作者单位： | 1.深圳出入境检验检疫局，深圳 518010；
2.中国科学院遗传与发育生物学研究所，北京100101；
3.华南农业大学，广州 510642
1.Shenzhen Exit-Entry Inspection and Quarantine Bureau,Shenzhen 518100 China;
2.Institute of Genetics and Developmental Biology,Chinese Academy of Sciences,Beijing 100101,China;
3.South China Agricultural University,Guangzhou 510642 China |
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| 基金项目： | 中国国家出入境检验检疫局 2 0 0 0年科学研究与技术开发项目 (K0 40 -2 0 0 0 ) |
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| 摘 要： | 采用PCR检测方法从饲料的主要原料豆粕、玉米蛋白粉中成功地检出启动子35S (35S-promoter,originated from cauliflower mosaic virus)、终止子NOS (nopaline synthase-terminator,derived from Agrobacterium tumefaciens)、耐除草剂基因EPSPS(5-enolpyruvylshikimate-3-phosphate synthase)和抗虫基因CryIA（b）(delta-endotoxin,evolved from Bacillus thuringiensis subsp.kurstaki)等转基因成分，并通过扩增玉米自身蛋白基因Zein( a protein extracted from corn gluten)及大豆自身基因Lectin(chitin-binding protein)的引物和阴阳性对照、阴阳性质控，避免假阳性、假阴性结果。该方法已在口岸进口饲料原料转基因检测中得到初步应用。
Abstract:Based on the heterogenous genes usually used in transgenic crops,the PCR technique was performed with primers derived from CaMV 35S promoter(35S-promoter,originated from cauliflower mosaic virus),NOS terminator(nopaline synthase-terminator,derived from Agrobacterium tumefaciens),EPSPS(5-enolpyruvylshikimate-3-phosphate synthase) gene,and CryIA(b)(delta-endotoxin,evolved from Bacillus thuringiensis subsp.kurstaki)gene to detect transgenic agents from feed raw materials of soybean dregs and corn gluten meal,respectively.Endogenous corn Zein(a protein extracted from corn gluten) gene,soybean Lectin(chitin-binding protein) gene and negative,positive control were applied for avoiding false results.The method established here has been succeessfully applied in detecting transgenic elements in imported feed raw material.
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| 关 键 词： | 饲料 豆粕 玉米蛋白粉 Key words 转基因 PCR |
| 文章编号： | 0253-9772(2002)03-0293-04 |
| 修稿时间： | 2001年8月13日 |
PCR Detection of Transgenic Elements in Feed Raw Material |
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| WANG Ying ,YU Dao jian ,KANG Lin ,ZHANG Gui ming ,JIN Xian zhong ,YANG Wei dong ,HUANG Pei qing ,WU Qiong ,CHEN Zhi nan ,CHU Cheng cai ,CHENG Ying hui.PCR Detection of Transgenic Elements in Feed Raw Material[J].Hereditas,2002,24(3):293-296. |
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| Authors: | WANG Ying YU Dao jian KANG Lin ZHANG Gui ming JIN Xian zhong YANG Wei dong HUANG Pei qing WU Qiong CHEN Zhi nan CHU Cheng cai CHENG Ying hui |
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| Institution: | Shenzhen Exit-Entry Inspection and Quarantine Bureau, Shenzhen 518100, China. wy-liza@21cn.com |
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| Abstract: | Based on the heterogenous genes usually used in transgenic crops,the PCR technique was performed with primers derived from CaMV 35S promoter(35S-promoter,originated from cauliflower mosaic virus), NOS terminator(nopaline synthase-terminator,derived from Agrobacterium tumefaciens),EPSPS (5-enolpyruvylshikimate-3-phosphate synthase) gene,and CryIA(b) (delta-endotoxin,evolved from Bacillus thuringiensis subsp.kurstaki)gene to detect transgenic agents from feed raw materials of soybean dregs and corn gluten meal,respectively.Endogenous corn Zein (a protein extracted from corn gluten) gene,soybean Lectin (chitin-binding protein) gene and negative,positive control were applied for avoiding false results.The method established here has been succeessfully applied in detecting transgenic elements in imported feed raw material. |
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| Keywords: | PCR transgene feed raw material soybean dreg corn gluten meal |
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