云南保山猪线粒体DNA D-loop区序列初步分析 Primary Analysis of Mitochondrial DNA D-loop Region Sequence in Baoshan Pig

摘要：为了解云南保山猪(Baoshan pig)的遗传多样性及其遗传背景，我们测定了19个个体线粒体DNA D-loop高变区I 15 363～15 801片段序列438 bp。检测到10种单倍型，包括8个多态位点，其中5次T/C转换、1次G/A转换、1次G/C颠换和1次A/T颠换，其A、T、G、C碱基的平均含量分别为35.4%、26.9%、13.2%和24.5%，A+T含量(62.3%)明显高于G+C含量(37.7%)。对于保山猪的保种及其持续利用有着重要的理论指导意义。 Abstract:To investigate the genetic diversity and genetic data of Baoshan pig in Yunnan province,the mitochondrial DNA D-loop hypervariable segment I sequences 15 363～15 801 (438 bp) in 19 individuals of Baoshan pig were sequenced.Ten mitochondrial haplotypes were identified in the samples,with 8 sites showing polymorphism,which were 5 T/C and 1 G/A transitions,1 G/C and 1 A/T transversions.The contents of A,T,G and C were 35.4%,269%,13.2% and 24.5%,respectively.The content of A+T (62.3%) was significantly higher than that of G+C (37.3%).It will be of importance to conservation and sustainable utilization in Baoshan pig.

Primary Analysis of Mitochondrial DNA D-loop Region Sequence in Baoshan Pig

To investigate the genetic diversity and genetic data of Baoshan pig in Yunnan province,the mitochondrial DNA D-loop hypervariable segment I sequences 15 363 approximately 15 801 (438 bp) in 19 individuals of Baoshan pig were sequenced. Ten mitochondrial haplotypes were identified in the samples, with 8 sites showing polymorphism, which were 5 T/C and 1 G/A transitions, 1 G/C and 1 A/T transversions. The contents of A,T,G and C were 35.4%,26 9%,13.2% and 24.5%,respectively. The content of A+T (62.3%) was significantly higher than that of G+C (37.3%). It will be of importance to conservation and sustainable utilization in Baoshan pig.