| 黄鳝Hprt基因的克隆及表达分析 |
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| 引用本文： | 何焱,商璇,程汉华,周荣家.黄鳝Hprt基因的克隆及表达分析[J].遗传,2006,28(6):677-682. |
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| 作者姓名： | 何焱 商璇 程汉华 周荣家 |
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| 作者单位： | 武汉大学生命科学学院 发育生物学研究中心， 武汉 430072 |
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| 基金项目： | 中国科学院资助项目，科技部科研项目，新世纪优秀人才支持计划 |
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| 摘 要： | 次黄嘌呤鸟嘌呤磷酸核糖转移酶(Hprt)参与嘌呤核苷酸的补救合成。采用RACE技术克隆了黄鳝的次黄嘌呤鸟嘌呤磷酸核糖转移酶基因，它的全长cDNA 为1 452 bp，预测编码218个氨基酸，与人类、小鼠、鸡和斑马鱼等脊椎动物Hprt氨基酸序列之间的同源性超过76.7％。基于该基因氨基酸序列构建了进化树，显示与斑马鱼Hprt基因更同源。RT-PCR表明黄鳝Hprt基因在多种组织中广谱表达，表明黄鳝该基因在功能和进化上的保守性。
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| 关 键 词： | Hprt 性反转 突变 黄鳝 |
| 文章编号： | 0253-9772(2006)06-0677-06 |
| 收稿时间： | 2005-12-07 |
| 修稿时间： | 2006-01-13 |
Molecular Cloning and Expression Analysis of Swamp Eel (Monopterus albus) Hprt |
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| HE Yan,SHANG Xuan,CHENG Han-Hua,ZHOU Rong-Jia.Molecular Cloning and Expression Analysis of Swamp Eel (Monopterus albus) Hprt[J].Hereditas,2006,28(6):677-682. |
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| Authors: | HE Yan SHANG Xuan CHENG Han-Hua ZHOU Rong-Jia |
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| Institution: | Center for Developmental Biology, College of Life Sciences, Wuhan University, Wuhan 430072, China |
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| Abstract: | The enzyme hypoxanthine-guanine phosphoribosyltransferase(HPRT) plays an important role in the purine salvage pathways.Full-length cDNA of swamp eel(Monopterus albus,the rice field eel) Hprt gene was obtained by the RACE method.The cDNA sequence was 1 452 bp in length,which encoded a protein of 218 amino acids.Amino acid identities of Hprt between swamp eel and other vertebrates including human,mouse,chicken and zebrafish were more than 76.7%.Phylogenetic tree analysis based on the amino acid sequences showed that Hprt of swamp eel and zebrafish was in one clade.RT-PCR analysis showed ubiquitous expression pattern of swamp eel Hprt in adult tissues,suggesting that it has a constitutive function and is conserved during evolution. |
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| Keywords: | Hprt |
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