微卫星DNA标记分析德国镜鲤的遗传潜力

结合体重、体长、体高等数量性状, 用30个微卫星分子标记, 评估了3个德国镜鲤群体的遗传潜力, 共检测到287个等位基因, 559种基因型, 片段长度109~400 bp, 有效等位基因数1.1014~6.4665, 观察杂合度0.0968~0.9892, 期望杂合度0.0926~0.8554, 位点多态信息含量0.08787~0.8559, 其中中度多态(0.25≤PIC≤0.5)13个, 高度多态(PIC≥0.5)13个。统计结果显示: 3个群体的遗传潜力处于中度水平, 双来养殖群体的遗传潜力比换新和松浦繁殖群体低。同时, 用30个基因座的不同等位基因和基因型与双来群体的体重、体长、体高进行了连锁分析, 得到2个与镜鲤体长相关的微卫星分子标记(HLJ319, HLJ693)和1个与体高相关的位点(HLJ677), 与体长相关的1个位点(HLJ693)还与体重连锁。将此遗传标记在鲤鱼重组自交系中验证, 结果显示: 主要经济性状相连锁的3个遗传标记中HLJ319与鲤鱼体长性状的QTL定位结果基本一致。

Genetic potential analysis of Germany mirror carp (Cyprinus carpio L.) using microsatellite markers

Using 30 microsatellite markers and combining quantity characters such as body weight, body size and body width, we evaluated the genetic potential of 3 Germany mirror carp populations. Number of effective alleles (Ae), observed (Ho) and expected (He) heterozygosity values and polymorphic information contents (PIC) were all calculated. 287 alleles and 559 genotypes were detected. The DNA fragment length was 109-400 bp. The Hardy-Weinberg Equilibrium was checked and phenomenon of some disequilibrium were studied according to the test of c2. The result showed that the level of genetic variability was moderate, but genetic potential of Shuanglai population was much lower than that Huanxin and Songpu breeding populations. Polymorphic Information Contents (PIC) of the 3 populations of Germany mirror carp were between 0.08787 and 0.5377, both of highly and moderately polymorphic markers were 13. The number of effective alleles (Ae) were between 1.1014 and 6.4665. The observed (Ho) and expected (He) heterozygosity values were 0.0968-0.9892 and 0.0926-0.8554 respectively. The linkage correlation was analyzed using data of body weight, body size and body width and 30 loci. The result shown that there existed 2 loci, HLJ319 and HLJ693 associated with body size. The HLJ693 locus significantly correlated with body weight character. The HLJ677 locus linked with body width. And then the result were verified in Recombinant Inbred Lines (RIL) of common carp. It shown that the HLJ319 locus was significantly linked with body size, the same as the result of common carp QTL location.