首页 | 本学科首页   官方微博 | 高级检索  
   检索      

用RAPD方法对平菇、香菇属间原生质体融合子的研究
引用本文:刘国振,刘振岳,贾建航,刘丽娟,泰立芳,李小兵,朱衡,朱立煌LIU Guo-Zhen,LIU Zhen-Yue,JIA Jian-Hang,LIU Li-Juan,Tai Li-Fang,LI Xiao-Bing,ZHU Heng,ZHU Li-Huang.用RAPD方法对平菇、香菇属间原生质体融合子的研究[J].遗传,1995,17(5):37-40.
作者姓名:刘国振  刘振岳  贾建航  刘丽娟  泰立芳  李小兵  朱衡  朱立煌LIU Guo-Zhen  LIU Zhen-Yue  JIA Jian-Hang  LIU Li-Juan  Tai Li-Fang  LI Xiao-Bing  ZHU Heng  ZHU Li-Huang
作者单位:1.河北农业大学实验中心, 保定 071001 2.中国科学院遗传研究所,北京 100101 1.Technical Centre,Hebei Agricultural University,Baoding 071001 2.Institute of Genetics,Academia Sinica,Beiing 100101
摘    要:RAPD是一种新的分子生物学技术。本实验用28个随机引物对平菇、香菇及其融合子进行PCR扩增, 在融合子谱带中,有64条与平菇相同(与平菇的相似指数SI=70.6%),14条与香菇相同(SI=33.6%),另外有26条谱带为双亲与融合子所共有。本实验结果说明融合子是双亲融合的产物,但在融合过程中,从双亲获得的遗传信息不等。本实验首次在食用真菌中获得了原生质体融合的分子生物学证据。我们认为,在食用菌研究中,RAPD将是一种有重要价值的分析方法。 Abstract:RAPD is a novel molecular biological technique.P.sapidus,L.edodes and their intergeneric fusants were tested by RAPD method using 28 randomly selected primers.In bands gained from fusants by amplification reaction,there were 64 bands with the same position as in P.sapidus (the similarity index (SI) was 70.6%),14 bands as in L.edodes.This results illustrated that fusants are true hybrid derived from protoplast fusion procedure,but the genetic information obtained from the two parents were not equal.Molecular biological evidence of protoplast fusion had obtained first time in edible mushroom by RAPD method in this experiment,we suggest that this technique is a valuable genetic marker for mushroom study.

关 键 词:原生质体融合  RAPD  香菇  平菇  Key  words  Pleurotus  sapidus  

Studies on Fusants Dcrived from Intergeneric Protoplast Fusionof P.sapidus and L.edodes by RAPD Analysis
LIU Guo-Zhen,LIU Zhen-Yue,JIA Jian-Hang,LIU Li-Juan,Tai Li-Fang,LI Xiao-Bing,ZHU Heng,ZHU Li-Huang.Studies on Fusants Dcrived from Intergeneric Protoplast Fusionof P.sapidus and L.edodes by RAPD Analysis[J].Hereditas,1995,17(5):37-40.
Authors:LIU Guo-Zhen  LIU Zhen-Yue  JIA Jian-Hang  LIU Li-Juan  Tai Li-Fang  LI Xiao-Bing  ZHU Heng  ZHU Li-Huang
Abstract:
Keywords:
本文献已被 维普 等数据库收录!
点击此处可从《遗传》浏览原始摘要信息
点击此处可从《遗传》下载免费的PDF全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号