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罗氏沼虾不同群体线粒体COI基因的序列差异和遗传标记研究
引用本文:杨学明,郭亚芬,陈福艳,蒋钦杨,梁万文,蒋和生.罗氏沼虾不同群体线粒体COI基因的序列差异和遗传标记研究[J].遗传,2006,28(5):540-544.
作者姓名:杨学明  郭亚芬  陈福艳  蒋钦杨  梁万文  蒋和生
作者单位:1.广西水产研究所,南宁 530021; 2.广西大学动物科技学院,南宁 530005
摘    要:利用PCR方法扩增获得罗氏沼虾(Macrobrachiumrosenbergii)线粒体DNA的COI基因,测定该基因片段序列。分析了罗氏沼虾缅甸原种F1代、江苏养殖群体和广西选育F2代3个群体共17只个体的序列核苷酸位点差异和遗传多态。结果表明,缅甸原种F1代遗传多样性最为丰富,江苏养殖群体和广西选育群体的遗传多样性相对贫乏。在长度为498bp的基因片段中,共检测到10个多态性核苷酸位点(占2.01%),17只个体具有5种基因型,3群体各自的平均核苷酸位点差异分别为0.88%、0.07%和0。UPGMA分子系统聚类树显示,江苏养殖群体和广西选育群体的遗传关系最近,其单倍型混杂聚成一支,而缅甸原种F1群体相对独立为另外一支。COI基因可以作为区分两分支群体的遗传标记。

关 键 词:遗传标记  序列多态性  COI基因  罗氏沼虾  
文章编号:0253-9772(2006)05-0540-05
收稿时间:2005-05-31
修稿时间:2005-07-18

Intraspecific DNA Sequence Polymorphism and genetic markers in Mitochondrial COI Gene from Three Populations of Macrobrachium rosenbergii
YANG Xue-Ming,GUO Ya-Fen,CHEN Fu-Yan,JIANG Qin-Yang,LIANG Wan-Wen,JIANG He-Sheng.Intraspecific DNA Sequence Polymorphism and genetic markers in Mitochondrial COI Gene from Three Populations of Macrobrachium rosenbergii[J].Hereditas,2006,28(5):540-544.
Authors:YANG Xue-Ming  GUO Ya-Fen  CHEN Fu-Yan  JIANG Qin-Yang  LIANG Wan-Wen  JIANG He-Sheng
Institution:1.Guangxi Fisheries Institute, Nanning 530021,China; 2.College of Animal Science and Technology, Guangxi University, Nanning  530005, China
Abstract:The mitochondrial COI gene was PCR amplified and sequenced from 17 samples obtained from three populations of Macrobrachium rosenbergii (F1 of the Burma wildtype population, Jiangsu cultured population and F2 of the Guangxi breeding population). A 498-bp long partial gene segment was acquired and used to study the genetic diversity among the three populations. Results indicated that the COI gene locus was relatively more polymorphic in the F1 of Burma wildtype population, while the polymorphism in Jiangsu cultured population and Guangxi breeding population were very poor. A total of 10 polymorphic sites and 5 haplotypes were found in the sequences of the 17 samples. The average nucleotide divergence in the three populations was 0.88%0.07% and 0 respectively. The UPGMA phylogenetic tree suggested that F2 of the Guangxi breeding population and Jiangsu cultured population were closest genetically, and their haplotypes could be gathered together to a genetic branch while F1 of the Burma wildtype population diverged and could form another relatively independent branch. For these distinct nucleotide differences, COI gene could be suitable as a genetic marker for distinguishing these two branches of the Macrobrachium rosenbergii population.
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