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| 大腹园蛛大壶状腺丝蛋白-1基因部分cDNA的克隆和序列分析 | |
| 引用本文： | 任洪林,张文惠,潘风光,卢士英,柳增善,张俊辉.大腹园蛛大壶状腺丝蛋白-1基因部分cDNA的克隆和序列分析[J].遗传,2009,31(2):180-185. |
| 作者姓名： | 任洪林 张文惠 潘风光 卢士英 柳增善 张俊辉 |
| 作者单位： | 1. 人兽共患病教育部重点实验室,吉林大学人兽共患病研究所,长春,130062 2. 福建省亚热带植物研究所,厦门,361006 3. 吉林大学军需科技学院,长春,130062 |
| 摘 要： | 大腹园蛛大壶状腺表达拖丝蛋白新基因的克隆, 为进一步研究蛛丝蛋白基因以及人工表达蛛丝蛋白提供参考依据。文章利用“通用方法”即反转录—置换法构建大腹园蛛(Araneus ventricosus)大壶状腺(Major ampullate gland) cDNA文库, 并筛选出具有典型重复结构的大腹园蛛大壶状腺丝蛋白-1部分cDNA序列AvMaSp1 (GenBank登录号: AY177203)。该部分序列大小为1 408 bp, 编码区为1 288 bp, 编码氨基酸429个, 预测分子量为34.07 kDa, 典型的重复结构为 (GA)nAm(GA)N, 与十字园蛛(Araneus diadematus)丝蛋白基因ADF-1 (GenBank登录号: ADU47853)同源关系最近, 一致性为75.0%。 |
| 关 键 词： | 大腹园蛛 大壶状腺 蜘蛛丝 拖丝 蛛丝蛋白 |
| 收稿时间： | 2008-05-06 |
| 修稿时间： | 2008-09-04 |
Cloning and sequence analysis of major ampullate spidroin-1 partial cDNA from Araneus ventricosus |
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| REN Hong-Lin,ZHANG Wen-Hui,PAN Feng-Guang,LU Shi-Ying,LIU Zeng-Shan,ZHANG Jun-Hui.Cloning and sequence analysis of major ampullate spidroin-1 partial cDNA from Araneus ventricosus[J].Hereditas,2009,31(2):180-185. | |
| Authors: | REN Hong-Lin ZHANG Wen-Hui PAN Feng-Guang LU Shi-Ying LIU Zeng-Shan ZHANG Jun-Hui |
| Institution: | 1. Key Laboratory of Zoonosis, Ministry of Education, Institute of Zoonosis, Jilin University, Changchun 130062, China; 2. College of Light Industry and Economic, Jilin University, Changchun 130062, China; 3. Fujian Institute of Subtropical Botany, Xiamen 361006, China |
| Abstract: | In this paper, we successfully constructed the cDNA library of major ampullate gland of Araneus ventricousus using pUC18 vector and cloned the partial cDNA (AvMaSp1, GenBank accession number AY177203) encoding spider ma-jor ampullate gland spidroin-1 by means of picking colony randomly (Bird gun). The partial cDNA sequence of AvMaSp1 was 1 408 bp and its region in encoding was 1 288 bp. The protein deduced from AvMaSp1 contained 429 amino acid resi-dues and molecular weight was 34.07 kDa. The repetitive motif of this cDNA sequence was (GA)nAm(GA)N and the identity with A. diadematus fibroin-1 mRNA (ADF-1, GenBank accession number ADU47853) was 75.0%. |
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