| 胡萝卜与烟草原生质体的低温保存 |
| |
| 引用本文： | 赵世民,徐金相,刘根齐,刘承宪,李雪梅ZHAO Shi-Min,XU Jin-Xiang,LIU Gen-Qi,LIU Cheng-Xian,li Xue-Mei.胡萝卜与烟草原生质体的低温保存[J].遗传,1997,19(6):18-20. |
| |
| 作者姓名： | 赵世民 徐金相 刘根齐 刘承宪 李雪梅ZHAO Shi-Min XU Jin-Xiang LIU Gen-Qi LIU Cheng-Xian li Xue-Mei |
| |
| 作者单位： | 1.中国科学院遗传研究所,北京100101
2.中国科学院上海植物生理研究所,上海 200031
1.Institute of Genetics,The Chinese Academy of Sciences,Beijing 100101
2.Shanghai Institute of Plant Physiology,The Chinese Academy of Sciences,Shanghai 200031 |
| |
| 摘 要： | 在较长的时间内,可保持正常生理状态与细胞活性的原生质体的制备及保存技术是进行空间细胞融合实验的前提条件。本研究以0.7ml/L的葡萄糖作渗透压,在6-8℃低温条件下经过72小时保存后,供试原生质体的存活率仍可达81%,并保持了其长壁与分裂的特性。
Abstract:Storage duration and acativity of isolated protoplasts are important problem for experiment on plant cell fusion in space.We have achieved preliminary success using glucose as osmotic reagent,The survival protopasts after 72 hour storage in 0.7mol/L glucose at 6~8℃ reached 81% and maintained a very high activity of division.
|
| 关 键 词： | 胡萝卜 烟草 原生质体 低温保存 Key words Carrot 基因型 大麦 培养体系 直接培养 游离小孢子 |
Low Temperature Storage of the Protoplasts of Carrot and Tabacco |
| |
| ZHAO Shi-Min,XU Jin-Xiang,LIU Gen-Qi,LIU Cheng-Xian,li Xue-Mei.Low Temperature Storage of the Protoplasts of Carrot and Tabacco[J].Hereditas,1997,19(6):18-20. |
| |
| Authors: | ZHAO Shi-Min XU Jin-Xiang LIU Gen-Qi LIU Cheng-Xian li Xue-Mei |
| |
| Abstract: | |
| |
| Keywords: | Culture system Direct ulture Genotype Isolated microspore Barley |
| 本文献已被 维普 等数据库收录！ |
| 点击此处可从《遗传》浏览原始摘要信息 |
| 点击此处可从《遗传》下载免费的PDF全文 |
|
