小黑麦×小滨麦后代1RS·1BL易位系的选育和鉴定

利用细胞学、染色体C分带和原位杂交方法，对八倍体小黑麦劲松49与八倍体小滨麦杂种后代选育的稳定遗传材料山农030-1进行了鉴定。染色体观察结果表明，山农030-1根尖染色体数目为42，花粉母细胞减数分裂中期I(PMC MI)染色体构型为2ｎ=21Ⅱ。分别以黑麦、滨麦草基因组DNA为探针进行原位杂交，表明山农030-1含有一对黑麦和小麦的整臂易位染色体，不含有来自滨麦草的遗传物质。染色体C分带结果进一步表明此材料为1RS·1BL易位系。接种鉴定表明，山农030-1对白粉病免疫。Abstract: In the progenies of octoploid Triticale Jinsong49 crossed with octoploid Tritileymus, a stable germplasm line named Shannong030-1 was obtained. Its chromosome number was 42, and 21 bivalents could be observed at PMC MI. FISH analysis by using genomic DNAs from S. cereale and genomic DNAs from L. mollis as probes,respectively, showed that Shannong030-1 contained a pair of translocation chromosome between rye and wheat, and had no genetic materials from L. mollis . C-banding analysis showed that it was translocation lines of 1RS·1BL.Resistance identification showed Shannong030-1 was immune to powdery mildew.

Development and Molecular Cytogenetic Identification of 1RS·1BL Translocation lines derived from Triticale×Tritileymus

In the progenies of octoploid Triticale Jinsong49 crossed with octoploid Tritileymus, a stable germplasm line named Shannong 030-1 was obtained. Its chromosome number was 42, and 21 bivalents could be observed at PMC MI. FISH analysis by using genomic DNAs from S. cereale and genomic DNAs from L. mollis as probes, respectively, showed that Shannong 030-1 contained a pair of translocation chromosome between rye and wheat, and had no genetic materials from L. mollis. C-banding analysis showed that it was translocation lines of 1RS.1BL. Resistance identification showed Shannong 030-1 was immune to powdery mildew.