| B-RAF基因特异的siRNA干扰对胃癌BGC823细胞的影响 |
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| 引用本文： | 付浩,赵丹懿,孙秀菊,滑君,阎杨,邱广蓉,尚超,富伟能,孙开来.B-RAF基因特异的siRNA干扰对胃癌BGC823细胞的影响[J].遗传,2007,29(5):537-540. |
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| 作者姓名： | 付浩 赵丹懿 孙秀菊 滑君 阎杨 邱广蓉 尚超 富伟能 孙开来 |
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| 作者单位： | 1. 沈阳中国医科大学医学遗传教研室, 沈阳110001;2. 沈阳医学院生物化学教研室, 沈阳110046;3. 中国医科大学第一附属医院肿瘤外科,沈阳110001 ; |
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| 基金项目： | 国家自然科学基金资助项目(编号:30571836)~~ |
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| 摘 要： | 为探讨B-RAF基因特异的siRNA干扰对胃癌BGC823细胞的增殖和凋亡的影响, 设计并合成B-RAF小分子干扰RNA(B-RAF-siRNA)和阴性对照siRNA, 用TransMessenger介导转染胃癌BGC823细胞, RT-PCR分析检测胃癌BGC823细胞中B-RAF基因以及Bcl-2基因的表达; MTT检测胃癌BGC823细胞增殖情况; 流式细胞仪检测细胞凋亡情况, 并与对照组进行比较。TransMessenger能够有效介导B-RAF-siRNA和阴性对照siRNA转染胃癌BGC823细胞, TransMessenger介导的B-RAF-siRNA有效地抑制胃癌BGC823细胞B-RAF以及Bcl-2基因的表达, 与对照组相比, 抑制率达90.0%以上, 最高达100%; 同时明显抑制胃癌BGC823细胞增殖; 促进胃癌BGC823细胞的凋亡(P < 0.01)。B-RAF基因特异的siRNA干扰能有效地抑制胃癌BGC823细胞中B-RAF基因以及Bcl-2基因的表达, 同时促进胃癌细胞凋亡和抑制胃癌细胞增殖。
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| 关 键 词： | RNA干扰 B-RAF基因 胃癌细胞 细胞凋亡 |
| 文章编号： | 10.1360/yc-007-0537 |
| 收稿时间： | 2006-11-29 |
| 修稿时间： | 11 29 2006 12:00AM |
Effect of B-RAF-specific RNA interference on gastric cancer BGC823 cell line |
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| FU Hao,ZHAO Dan-Yi,SUN Xiu-Ju,HUA Jun,YAN Yang,QIU Guang-Rong,SHANG Chao,FU Wei-Neng,SUN Kai-Lai.Effect of B-RAF-specific RNA interference on gastric cancer BGC823 cell line[J].Hereditas,2007,29(5):537-540. |
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| Authors: | FU Hao ZHAO Dan-Yi SUN Xiu-Ju HUA Jun YAN Yang QIU Guang-Rong SHANG Chao FU Wei-Neng SUN Kai-Lai |
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| Institution: | 1. Department of Medical Genetics, China Medical University, Shenyang 110001, China;2. Department of Biochemistry, Shenyang Medical Colleg, Shenyang 110046, China;3. Department of Surgical Oncology, First Affiliated Hospital, China Medical University, Shenyang 110001, China ; |
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| Abstract: | To investigate the influence of B-RAF-specific RNA interference on the proliferation and apoptosis of gastric cancer BGC823 cell line. B-RAF-siRNA and scramble-siRNA were synthesized and transfected into BGC823 cells by TransMessenger. The expression of B-RAF gene and Bcl-2 gene in BGC823 cells was detected by RT-PCR and the level of apoptosis was evaluated in transfected cells by flow cytometry. Results showed that TransMessenger could effectively transfect B-RAF-siRNA and scramble-siRNA into BGC823 cells. B-RAF-siRNA significantly inhibited the expression of B-RAF gene and Bcl-2 gene in BGC823 cells by more than 90% to 100%. B-RAF-siRNA inhibited BGC823 cell prolifera- tion and induced apoptosis (P < 0.01). In conclusion, B-RAF-siRNA can effectively inhibit the expression of B-RAF gene and Bcl-2 gene, induce cell apoptosis and inhibit the proliferation of gastric cancer BGC823 cells. |
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| Keywords: | RNA interference B-RAF gene gastric cancer cell apoptosis |
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