小麦族鹅观草属、披碱草属、猬草属和仲彬草属细胞质基因组PCR-RFLP分析

对鹅观草属、披碱草属、猬草属和仲彬草属23个物种和1份外类群共24份材料进行了细胞质基因组PCR-RFLP分析。3个叶绿体和3个线粒体通用引物扩增出的片段，用15种限制性内切酶对其进行酶切。在47种引物/酶组合中，获得329条DNA片段，其中304条具有多态性，占92.4%。结果表明鹅观草属、披碱草属、猬草属和仲彬草属材料存在属间和种间多态性，遗传相似系数较高。聚类分析显示仲彬草属单独聚为一类，鹅观草属R. grandis、R. aristiglumis、R. elytrigioides、R. alashanica、R. magnicaespes聚为一类，R. caucasica、R. ciliaris、R. amurensis、R. japonensis聚在一起，5个披碱草属材料、3个猬草属材料和1个鹅观草属物种R. kamoji聚为一类。这些结果与前人对其进行的RAPD和RAMP分析的结果基本一致。仲彬草属作为属分类等级处理是恰当的，对于鹅观草属、披碱草属和猬草属的系统地位和一些物种的分类处理，有待进一步研究。

PCR-RFLP Analysis on Roegneria, Elymus, Hystrix and Kengyilia in Triticeae (Poaceae)

PCR-RFLP of Plasmon on 23 species of Roegneria,Elymus,Hystrix and Kengyilia and 1 outgroup(Triticum aestivum L.) was carried out.The segments amplified with 3 cpDNA and 3 mtDNA universal primers were digested by 15 restriction endonucleases.Among 329 bands produced,304 were polymorphic(92.4%).PCR-RFLP results showed that polymorphisms existed between the four genera and between the species in Roegneria,Elymus,Hystrix and Kengyilia,and genetic similarity coefficient was high.Roegneria grandis,R.aristiglumis,R.elytrigioides,R.alashanica and R.magnicaespes were grouped,while R.caucasica,R.ciliaris,R.amurensis and R.japonensis clustered together.Species of Kengyilia clustered independently.Five species of Elymus,3 of Hystrix and Roegneria kamoji grouped together.The results suggested Kengyilia was a valid genus.As more cytological and molecular data are available,the taxonomic status of Roegneria,Elymus and Hystrix will be recognized.