| 低背景、高分辨率PAGE简易银染法 |
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| 引用本文： | 高东,杜飞,朱有勇.低背景、高分辨率PAGE简易银染法[J].遗传,2009,31(6):668-673. |
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| 作者姓名： | 高东 杜飞 朱有勇 |
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| 作者单位： | 云南农业大学农业生物多样性应用技术国家工程研究中心, 农业生物多样性和控制病虫害教育部重点实验室, 云南省植物病理重点实验室, 昆明 650201 |
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| 基金项目： | 国家重点基础研究发展规划(973计划) |
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| 摘 要： | 聚丙烯酰胺凝胶电泳银染一直存在耗时长、步骤繁琐等缺陷, 是其批量应用的瓶颈。文章报道了一种低背景、高分辨率的PAGE简易银染方法, 该法在降低NaOH浓度和批量显带方面进行了有益的探索, 建立了节省时间、节约试材, 对批量显带尤为实用的简易银染法。
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| 关 键 词： | 显影 银染 聚丙烯酰胺凝胶 |
| 收稿时间： | 2008-11-04 |
| 修稿时间： | 2008-11-28 |
Low-background and high-resolution contracted silver-stained method in polyacrylamide gels electrophoresis |
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| GAO Dong,DU Fei,ZHU You-Yong.Low-background and high-resolution contracted silver-stained method in polyacrylamide gels electrophoresis[J].Hereditas,2009,31(6):668-673. |
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| Authors: | GAO Dong DU Fei ZHU You-Yong |
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| Institution: | The National Center for Agricultural Biodiversity, Ministry of Education Key Laboratory of Agricultural Biodiversity for Plant Disease Management, Key laboratory of Plant Pathology, Yunnan Agricultural University, Kunming 650201, China |
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| Abstract: | The disadvantage of time-consuming and fussy steps of conventional silver-stained method for polyacrylamide gels is evident, and has become the choke point in its application. In this paper, a low-background and high-resolution con-tracted silver-stained method for polyacrylamide gels was established. Compared with the conventional banding method, this contracted method has fewer steps and needs fewer reagents, in particular, lower NaOH concentration. It is rapid and economic, especially for mass silver-staining. |
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