新疆三种雅罗鱼属鱼类mtDNA D-loop多态性及起源分化分析

用PCR-RFLP技术，对新疆分布的准噶尔雅罗鱼、贝加尔雅罗鱼和高体雅罗鱼的mtDNA D-loop高变区约827bp进行了扩增，用ScaI、HinfI、AluI、DdeI 4种限制性内切核酸酶对56个样本的扩增产物酶切和RFLP分析，共检测到6种单倍型。准噶尔雅罗鱼存在2种单倍型：BDAA和BDBA；贝加尔雅罗鱼存在3种：AAAA、ABAA和ACAA；高体雅罗鱼只有1种。初步认为，准噶尔雅罗鱼、贝加尔雅罗鱼存在较丰富的群体内变异。用6种单倍型及净遗传距离绘制的UPGMA分子系统树，提示准噶尔雅罗鱼有可能是原始种，贝加尔雅罗鱼和高体雅罗鱼是由准噶尔雅罗鱼进化而来。 Abstract:PCR-RFLP technique was employed to amplify about 827bp of mtDNA D-loop hypervariable region of Leuciscus baicalensis,L.merzbacheri and L.idus in Xinjiang.The PCR products of 56 samples with four restriction enzymes were digested:ScaI,HinfI,AluI,DdeI,and RFLP analysis was done then.The study indicates that all of L. species and populations have six haplotypes,L.merzbacheri has two haplotypes:BDAA,BDBA;L.baicalensis has three:AAAA,ABAA,ACAA;L.idus has one:CAAA.It is primarily considered that L.merzbacheri and L.baicalensis have more intra-population mutations.The UPGMA trees with 6 haplotypes and net genetics distance pointed out that L.merzbacheri might be original species,L.baicalensis and L.idus are evolved from L.merzbacheri.

Polymorphism and Original Analysis of mtDNA D-loop of Three Leuciscus Species in Xinjiang

PCR-RFLP technique was employed to amplify about 827bp of mtDNA D-loop hypervariable region of Leu-ciscus baicalensis ,L. merzbacheri and L. idus in Xinjiang. The PCR products of 56 samples with four restriction enzymes were digested:Seal, HintI,AluI,Ddel,and RFLP analysis was done then. The study indicates that all of L. species and populations have six haplotypes, L. merzbacheri has two haplotypes: BDAA, BDBA; L. baicalensis has three: AAAA, ABAA, ACAA; L. idus has one:CAAA. It is primarily considered that L. merzbacheri and L. baicalensis have more intra-population mutations. The UPGMA trees with 6 haplotypes and net genetics distance pointed out that L. merzbacheri might be original species,L. baicalensis and L. idus are evolved from L. merzbacheri.