| 双价抗虫基因叶绿体共转化植株抗虫性及其后代表型分析 |
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| 引用本文： | 苏宁,孙萌,杨波,孟昆,刘春英,倪丕冲,沈桂芳.双价抗虫基因叶绿体共转化植株抗虫性及其后代表型分析[J].遗传,2002,24(3):288-292. |
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| 作者姓名： | 苏宁 孙萌 杨波 孟昆 刘春英 倪丕冲 沈桂芳 |
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| 作者单位： | 1.中国农业科学院生物技术研究所叶绿体基因工程实验室，北京 100081;
2.浙江大学生命科学院，杭州 310027
1.Biotechnology Research Institute,CAAS,Beijing 100081;
2.College of Biology,Zhejiang University Hangzhou 310027,China |
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| 基金项目： | 国家自然科学基金资助 ( 395 70 36 1) |
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| 摘 要： | 利用基因枪法将含有水稻巯基蛋白酶抑制剂(Oryzacystatin,OC)基因烟草叶绿体表达载体和含有苏云金芽孢杆菌晶体毒蛋白基因(Bt cry IAc)烟草叶绿体表达载体,共转化烟草叶绿体,获得壮观霉素抗性植株。转基因植株抗棉铃虫试验表明,转双价抗虫基因植株比转单一抗虫基因植株具有更强的杀虫活性。转基因植株后代Southern检测及其遗传学分析试验证明,双价抗虫基因可以稳定地遗传给后代,且表现为叶绿体特有的母系遗传规律。
Abstract:The Bt gene and OC gene were cotransformed to tobacco chloroplast with particle bombardment method and spectinomycin resistance tobacco seedlings were obtained.Bioassays showed that the transgenic tobacco containing both genes had enhanced toxicity to the larvae of cotton bollworm (helicoverpa zea) by comparison with the plants containing only Bt or OC gene.Southern-blotting analysis and genetic analysis of progenies showed that the Bt and OC gene expressed and was inherited maternally to the progenies.
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| 关 键 词： | 抗虫作用 Key words OC gene 叶绿体转化 Bt基因 OC基因 |
| 文章编号： | 0253-9772(2002)03-0288-05 |
| 修稿时间： | 2001年9月19日 |
The Insect Resistance of OC and Bt Transplastomic Plant and the Phenotype of Their Progenies |
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| SU Ning ,SUN Meng ,YANG Bo ,MENG Kun ,LIU Chun ying ,NI Pi chong ,SHEN Gui fang.The Insect Resistance of OC and Bt Transplastomic Plant and the Phenotype of Their Progenies[J].Hereditas,2002,24(3):288-292. |
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| Authors: | SU Ning SUN Meng YANG Bo MENG Kun LIU Chun ying NI Pi chong SHEN Gui fang |
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| Institution: | Biotechnology Research Institute, CAAS, Beijing 100081, China. sn68@sina.com |
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| Abstract: | The Bt gene and OC gene were cotransformed to tobacco chloroplast with particle bombardment method and spectinomycin resistance tobacco seedlings were obtained.Bioassays showed that the transgenic tobacco containing both genes had enhanced toxicity to the larvae of cotton bollworm (helicoverpa zea) by comparison with the plants containing only Bt or OC gene.Southern-blotting analysis and genetic analysis of progenies showed that the Bt and OC gene expressed and was inherited maternally to the progenies. |
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| Keywords: | OC gene Bt gene chloroplast transformation insect resistance |
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