| 大肠埃希菌氨基糖苷类耐药株 Aac(3) Ⅱ 基因保守区分析 |
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| 引用本文： | 陈接根,吕建新,楼永良,彭颖,陈秀枢,陆永绥.大肠埃希菌氨基糖苷类耐药株 Aac(3) Ⅱ 基因保守区分析[J].遗传,2004,26(2):202-204. |
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| 作者姓名： | 陈接根 吕建新 楼永良 彭颖 陈秀枢 陆永绥 |
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| 作者单位： | 聚合酶链反应; DNA测序; 耐药基因; 氨基糖苷类抗生素; 钝化酶 Key words： PCR; DNA sequencing; Drug resistance gene; Aminoglycoside; Modifying enzyme |
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| 基金项目： | 国家高技术研究发展计划(863计划) |
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| 摘 要： | 常规方法分离鉴定47株大肠埃希菌，以标准纸片扩散法(K B法)对其进行常用氨基糖苷类药物敏感性测定，耐药株经PCR检测aac(3) Ⅱ基因保守区，扩增产物进行DNA测序分析。初步探讨大肠埃希菌氨基糖苷类抗生素耐药株与aac(3) Ⅱ基因保守区之间的关系，结果显示本地区大肠埃希菌氨基糖苷类抗生素耐药株的aac(3) Ⅱ基因保守区具65位G、84位T和65位A、84位C两种基因型，且高度耐药菌株皆为65位G、84位T基因型。Abstract:According to standard K B method,bacteriostatic tests were performed to screen out aminoglycoside resistance bacteria from 47 strains of isolated E.coli.To analyze correlations between the degree of E.coli aminoglycoside resistance and aac(3) Ⅱgene conserved region,PCR amplified aac(3) Ⅱgene conserved regions and were analyzed by DNA sequencing.The results showed that there were two species of aac(3) Ⅱgene type including 65G and 84T or 65A and 84C in the samples.Strains with high activity of modifying enzyme to gentamicin all were 65G and 84T aac(3) Ⅱgene type.
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| 关 键 词： | 温州医学院检验与公共卫生学院 细胞与分子医学研究所 Wenzhou Medical College Institute of Cellular and Molecular Medicine 温州 325027 School of Clinical Laboratory Medicine and Public Health |
| 文章编号： | 0253-9772(2004)02-0202-03 |
| 修稿时间： | 2003年6月30日 |
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