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RNA干涉(RNAi)技术应用于哺乳动物细胞的研究策略
引用本文:张定校,樊斌,刘榜,李奎.RNA干涉(RNAi)技术应用于哺乳动物细胞的研究策略[J].遗传,2005,27(5):839-844.
作者姓名:张定校  樊斌  刘榜  李奎
作者单位:1. 华中农业大学动物科技学院动物分子生物学与育种实验室,武汉430070;
基金项目:国家自然科学基金(编号:30300250),国家重点基础研究发展规划(编号:G2000016103),“863”项目(编号:2004AA222170)~~
摘    要:RNAi作为新近发展起来的基因功能分析技术,近年来在哺乳动物细胞中的研究已取得了长足进展,且有着广泛的应用前景。对RNAi作用机制及RNAi实验操作技术的探讨是目前研究的热点。研究表明,哺乳动物细胞中的RNAi作用模式与植物有所不同。文章对RNAi作用机制、哺乳动物细胞RNAi实验的一般策略(包括靶siRNA序列选择、siRNA获取方法、siRNA转染、RNAi效果检测等)以及最新研究进展进行简述,以供类似工作的参考。

关 键 词:基因功能分析  哺乳动物细胞  RNA干扰  siRNA  
文章编号:0253-9772(2005)05-0839-06
收稿时间:2004-08-29
修稿时间:2004-12-17

Experimental Strategies of the Application of RNAi Technique in Mammalian Cells
ZHANG Ding-Xiao,FAN Bin,LIU Bang,LI Kui.Experimental Strategies of the Application of RNAi Technique in Mammalian Cells[J].Hereditas,2005,27(5):839-844.
Authors:ZHANG Ding-Xiao  FAN Bin  LIU Bang  LI Kui
Institution:1. Laboratory of Molecular Biology and Animal Breeding, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, China; 2. Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100094, China
Abstract:As a novel and effective tool for gene function analysis, RNA interference (RNAi) technique has been developed rapidly and made great progress in mammalian cell studies. The mechanisms and experimental operation of RNAi in mammalian cells have become research hotspots, and RNAi mechanisms were supposed to be different from those in plant. In this paper, a review of RNAi mechanisms, the latest research progress and the experimental strategies of RNAi in mammalian cells were presented, including siRNA sequence design, siRNA delivery approaches and RNAi effect detection, so as to offer valuable references for RNAi studies.
Keywords:RNA interference  siRNA  gene function analysis  mammalian cells
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