DNA提取的应用与相关技术分析

为了分析影响提取DNA的有关因素，采用标准酚—氯仿抽提法和蛋白酶消化法提取DNA。结果表明，平均每mL全血可提取200 ~ 300μgDNA；新鲜组织及OCT包埋冰冻组织提取DNA，平均每0.2g组织可获得200~300μgDNA；直接将石蜡标本提取物制作模版，PCR扩增良好。从4种组织中均获得较为纯净的DNA；OCT对组织DNA无不良影响。 Abstract:To explore influence factors of DNA extraction,the protease and phenol-chloroform method was used to extract DNA in whole blood,fresh tissues,frozen tissues embedding with OCT and tissues embedding in paraffin.It results that 200~300μg DNA was extracted from 1ml whole blood or 0.2g fresh tissues or frozen tissue embedding with OCT.DNA extracted from paraffin specimen can be directly used in PCR amplification.Purity DNA can be extracted from four kinds of different tissues.OCT hasn't harmful effect on tissue DNA extraction.

Application of DNA Extraction and Analysis of Its Influence Factors

To explore influence factors of DNA extraction, the protease and phenol-chloroform method was used to extract DNA in whole blood, fresh tissues, frozen tissues embedding with OCT and tissues embedding in paraffin. It results that 200-300 microg DNA was extracted from 1 ml whole blood or 0.2 g fresh tissues or frozen tissue embedding with OCT. DNA extracted from paraffin specimen can be directly used in PCR amplification. Purity DNA can be extracted from four kinds of different tissues. OCT hasn't harmful effect on tissue DNA extraction.