微卫星DNA监控大鼠近交系的培育

采用微卫星DNA技术来监控大鼠仔代基因状况，选择性地进行交配繁殖，使基因快速纯合，缩短培育新的近交系动物周期。利用PCR扩增30个微卫星DNA位点对封闭群SD和Wistar大鼠交配繁殖的仔代鼠进行微卫星DNA多态性分析，仔代中与母代SD大鼠相似系数高的与中的进行定向交配繁殖。F2代大鼠均为杂合多态的位点，没有纯合位点；到F9代时基因纯合位点达27个，纯合基因位点率为90%。每代相似系数具有不断上升的趋势，上升率为6-20%。采用皮肤移植方法验证了F9代大鼠间无排斥。建立了一种新的快速培育近交系动物的方法。

Inbred Cultivation of Microsatellite DNA Monitoring Outbred Rats

Microsatellite DNA was used to monitor the gene inheritance of successive generations of rats.It is useful in selective breeding to quickly bring genes to homozygosity and to shorten the cultivation periods of new inbreeding of rats.Thirty microsatellite DNA loci were PCR amplified to analyze the DNA polymorphism in generational crosses between the outbred Wistar and SD rats.Rats in F generations with a moderate to high coefficient of similarithy with the female F_0 SD rats were crossed.All loci in the F_2 generation were heterozygous and polymorphic.By F_9,27 loci have become homozygotic,with a homozygosity rate of 90%.The coefficient of similarity tended to increase at a rate of 6%~20% with each successive generation.Skin grafting confirmed no rejection among the F_9 generation of rats.Therefore we have established a novel method for rapid cultivation of inbred rats.