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基于EST的新基因克隆策略
引用本文:刘媛,蔡嘉斌,蒋国松,童强松.基于EST的新基因克隆策略[J].遗传,2008,30(3):257-262.
作者姓名:刘媛  蔡嘉斌  蒋国松  童强松
作者单位:1. 华中科技大学同济医学院附属协和医院小儿外科, 武汉 430022;2. 华中科技大学同济医学院附属协和医院泌尿外科, 武汉 430022 ;
摘    要:表达序列标签(expressed sequence tags, EST) 是从随机选择的cDNA 克隆进行单向测序获得的短的cDNA序列, 代表一个完整基因的一部分。随着生物信息学和基因定位的迅猛发展, EST已成为基因定位、基因克隆、基因表达分析的有力工具。近年来, 由于EST数据库的迅速扩张, 运用EST来克隆和定位基因, 使得新基因克隆的策略发生了革命性变革。尽管存在一些不足, 实践证明EST可大大加速新基因的发现与研究。本文将就EST技术尤其是它在新基因克隆中的应用策略作详细介绍。

关 键 词:表达序列标签  基因克隆  基因  
收稿时间:2007-08-19
修稿时间:2007年8月19日

Strategies for cloning of novel genes based on EST
LIU Yuan,CAI Jia-Bin,JIANG Guo-Song,TONG Qiang-Song.Strategies for cloning of novel genes based on EST[J].Hereditas,2008,30(3):257-262.
Authors:LIU Yuan  CAI Jia-Bin  JIANG Guo-Song  TONG Qiang-Song
Institution:1. Department of Pediatric Surgery, Union Hospital Affiliated to Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China ;2. Department of Urology, Union Hospital Affiliated to Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China ;
Abstract:Expressed sequence tags (EST) are short, randomly selected single-pass nucleotide sequence reads derived from cDNA libraries and represent a small part of a gene. Along with the development of bioinformatics and genetic localization, EST has already become a powerful tool for mapping, cloning and expression profiling of genes. Recently, because of the fast distension of EST databases, application of EST in gene mapping and cloning leads to revolutionary change in the strategies for cloning of novel genes. Despite of some insufficiencies, it has been proved that EST could promote the discovery and research of novel genes. In this article, an introduction about EST, especially EST-based strategies for cloning of novel genes will be given in details.
Keywords:
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