| 转基因动物整合位点的研究进展 |
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| 引用本文： | 吴波,朱作言.转基因动物整合位点的研究进展[J].遗传,2003,25(1):77-80. |
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| 作者姓名： | 吴波 朱作言 |
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| 作者单位： | 中国科学院水生生物研究所淡水生态与生物技术国家重点实验室，武汉 430072 |
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| 基金项目： | 国家"973"项目(G2000016109,G2001CB109006)，国家自然科学基金重点项目(NSFC39823003)资助 |
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| 摘 要： | 转基因动物整合位点克隆及相关序列特征研究是探索外源基因整合机制和整合稳定性的重要手段。转基因整合位点序列的克隆方法主要有：个体基因组文库筛选法、反向PCR法、热不对称交互式PCR法和质粒回收法。4种方法各有优缺点，可根据不同条件选用。克隆到的整合位点序列表明，整合位点可能存在一定的共同特征。
Integration Sites of Transgenes in Transgenic Animals
WU Bo,ZHU Zuo-Yan
State Key Laboratory of Freshwater Ecology and Biotechnology,Institute of Hydrobiology,
Chinese Academy of Science,Wuhan 430072,China
Abstract:To study the mechanism of transgene ' s integration in the host genome,first step is to clone the integration-site sequence.The method employed for this purpose includes selection from genomic pool,IPCR,TAIL-PCR and plasmid rescued.Different method fits different cases depending on how the transgenics have been produced.The data of integration-site sequences showed some similar features existed.
Key words：transgene; integration site; clone method
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| 关 键 词： | 转基因 整合位点 克隆方法 |
| 文章编号： | 0253-9772(2003)01-0077-04 |
| 修稿时间： | 2002年4月15日 |
Integration Sites of Transgenes in Transgenic Animals |
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| Bo Wu,Zuo-Yan Zhu.Integration Sites of Transgenes in Transgenic Animals[J].Hereditas,2003,25(1):77-80. |
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| Authors: | Bo Wu Zuo-Yan Zhu |
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| Institution: | State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Science, Wuhan 430072, China. wuboy530@sohu.com |
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| Abstract: | To study the mechanism of transgene's integration in the host genome, first step is to clone the integration-site sequence. The method employed for this purpose includes selection from genomic pool, IPCR, TAIL-PCR and plasmid rescued. Different method fits different cases depending on how the transgenics have been produced. The data of integration-site sequences showed some similar features existed. |
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| Keywords: | transgene integration site clone method |
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