| 牛血清白蛋白在植物RAPD分析中的作用 |
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| 引用本文： | 边才苗,李钧敏,金则新,葛明菊.牛血清白蛋白在植物RAPD分析中的作用[J].遗传,2002,24(3):279-282. |
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| 作者姓名： | 边才苗 李钧敏 金则新 葛明菊 |
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| 作者单位： | 台州师范专科学校生化系，浙江临海 317000
Biology and Chemistry Department,Taizhou Teachers College,Linhai,Zhejiang 317000,China |
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| 基金项目： | 浙江省自然科学基金项目 ( 3992 0 3)，浙江省教委科研计划项目 ( 1990 36 7) |
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| 摘 要： | 以水杉、七子花DNA为模板，添加牛血清白蛋白（BSA），观察其对植物RAPD扩增效果的改善情况。研究显示，在水杉及七子花RAPD扩增体系中，改善RAPD扩增反应的最佳的BSA浓度是不同的，分别为06μg/μl与1μg/μl。另外，BSA还可以封闭乙酰BSA对RAPD扩增反应的抑制作用，降低RAPD反应系统中Taq酶的用量。
Abstract:Using Metasequoia glyptostroboides and Heptacodium miconioides DNA as templates,the effect of bovine serum albumin(BSA) on RAPD in plants was studied.The results showed that suitable concentrations of BSA used in Metasequoia glyptostroboides and Heptacodium miconioides RAPD were different,which were 06μg/μl and 1μg/μl,respectively.The inhibition of acetylated BSA on the amplification of plant RAPD could be relieved by BSA.BSA could reduce the dosage of Taq DNA polymerase.
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| 关 键 词： | 牛血清白蛋白 水杉 bovine serum albumin 七子花 RAPD Key words |
| 文章编号： | 0253-9772(2002)03-0279-04 |
| 修稿时间： | 2001年10月8日 |
Effect of BSA on Random Amplified Polymorphic DNA(RAPD) in Plants |
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| BIAN Cai miao,LI Jun min,JIN Ze xin,GE Ming ju.Effect of BSA on Random Amplified Polymorphic DNA(RAPD) in Plants[J].Hereditas,2002,24(3):279-282. |
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| Authors: | BIAN Cai miao LI Jun min JIN Ze xin GE Ming ju |
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| Institution: | Biology and Chemistry Department, Taizhou Teachers College, Linhai, Zhejiang 317000, China. lijm@mail.tzptt.zj.cn |
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| Abstract: | Using Metasequoia glyptostroboides and Heptacodium miconioides DNA as templates,the effect of bovine serum albumin (BSA) on RAPD in plants was studied. The results showed that suitable concentrations of BSA used in Metasequoia glyptostroboides and Heptacodium miconioides RAPD were different, which were 0.6 microg/microl and 1 microg/microl, respectively. The inhibition of acetylated BSA on the amplification of plant RAPD could be relieved by BSA. BSA could reduce the dosage of Taq DNA polymerase. |
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| Keywords: | bovine serum albumin Metasequoia glyptostroboides Heptacodium miconioides RAPD |
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