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泰和乌骨鸡的核型与带型研究
引用本文:陈国宏,刘莉,张学余,李碧春,吴信生,徐琪.泰和乌骨鸡的核型与带型研究[J].遗传,2003,25(4):401-408.
作者姓名:陈国宏  刘莉  张学余  李碧春  吴信生  徐琪
作者单位:1.扬州大学畜牧兽医学院动科系,江苏 扬州 225009; 2.江苏省家禽科学研究所,江苏 扬州 225003 1.Dept of Anim Sci,Anim Sci and Med Coll,Yangzhou Uni,Yangzhou 225009,China; 2.Jiangsu Provincial Institute of Poultry Science,Yangzhou 225003,China
基金项目:国家高新技术发展计划项目(863—2001AA243083),国家自然科学基金(30170673)
摘    要:采用外周血淋巴细胞培养--空气干燥法,对泰和乌骨鸡染色体核型和带型进行了研究。结果表明:泰和乌骨鸡体细胞染色体数目2n=78,染色体基本臂数AF=90,1、9号染色体及Z、W性染色体为中央着丝粒(m)染色体,2、4、7号染色体为亚中央着丝粒(sm)染色体,3、6、8、10号染色体为端着丝粒(t)染色体。G带研究表明:前10对大型染色体可分为29个区,190条带。C带处理发现,所有母鸡分裂相中W性染色体都出现C带并整条深染。Ag-NORs研究发现:Ag-NORs常定位于1、2号常染色体短臂和Z性染色体短臂端部;Ag-NORs数目分布范围为1~6;平均每个细胞的Ag-NORs数在雌、雄鸡中分别为2.94和2.96。 Abstract:This study made the chromosome slides of Taihe Silkies by the peripheral blood lymphocyte culture-drying method,and analyzed Taihe Silkies karyotype and band pattern.The results are as follow:The diploid chromosome number of Taihe Silkies was 2n=78,the basic number of chromosome arms was AF=90 and the sex chromosome type was ZZ(♂)/ZW(♀).According to the measured relative length,arm ratio and centromeric index,the first 10 pairs of macro-chromosomes are described as follows:No.1 ,9 and Z,W chromosomes were metacentrics,No.2,4,7 were submetacentrics,and No.3,6,8,10 were telocentrics.Studies on Taihe Silkies′ G-band showed that the first 10 pairs of macro-chromosomes can be divided into 29 zones and 190 bands.Being treated by C-banding technique,a totally dark-stained and easily indentified W-chromosome always showed up in the female metaphase configurations.Ag-NORs were located in the short arms′ telomere of No.1,2 euchromosomes and Z sexchromosome,the Ag-NORs number varied from 1-6. -NORs

关 键 词:测序  基因组  日本扁柏  微卫星  Ag-NORs  Key  words  C带  G带  泰和乌骨鸡  核型  
文章编号:0253-9772(2003)04-0401-08
修稿时间:2002年7月3日

Studies on Taihe Silkies′Karyotype and Band Pattern
CHEN Guo-Hong,LIU Li,ZHANG Xue-Yu,LI Bi-Chun,WU Xin-Sheng,XU Qi.Studies on Taihe Silkies′Karyotype and Band Pattern[J].Hereditas,2003,25(4):401-408.
Authors:CHEN Guo-Hong  LIU Li  ZHANG Xue-Yu  LI Bi-Chun  WU Xin-Sheng  XU Qi
Institution:Dept of Anim Sci, Anim Sci and Med Coll, Yangzhou Uni, Yangzhou 225009, China.
Abstract:This study made the chromosome slides of Taihe Silkies by the peripheral blood lymphocyte culture-drying method,and analyzed Taihe Silkies karyotype and band pattern. The results are as follow: The diploid chromosome number of Taihe Silkies was 2n=78,the basic number of chromosome arms was AF=90 and the sex chromosome type was ZZ(male symbol)/ZW(female symbol). According to the measured relative length,arm ratio and centromeric index,the first 10 pairs of macro-chromosomes are described as follows:No.1 ,9 and Z,W chromosomes were metacentrics,No.2,4,7 were submetacentrics,and No.3,6,8,10 were telocentrics. Studies on Taihe Silkies' G-band showed that the first 10 pairs of macro-chromosomes can be divided into 29 zones and 190 bands. Being treated by C-banding technique,a totally dark-stained and easily indentified W-chromosome always showed up in the female metaphase configurations. Ag-NORs were located in the short arms' telomere of No.1,2 euchromosomes and Z sexchromosome,the Ag-NORs number varied from 1-6. -NORs.
Keywords:Taihe Silkies  karyotype  G-banded pattern  C-banded pattern  Ag-NORs
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