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改良CTAB法用于多年生植物组织基因组DNA的大量提取*
引用本文:陈昆松,李方,徐昌杰,张上隆,傅承新.改良CTAB法用于多年生植物组织基因组DNA的大量提取*[J].遗传,2004,26(4):529-531.
作者姓名:陈昆松  李方  徐昌杰  张上隆  傅承新
作者单位:1. 浙江大学果实生理与生物技术实验室/农业部园艺植物生长发育与生物技术重点开放实验室,华家池校区, 杭州 310029; 2. 浙江大学生命科学学院,华家池校区, 杭州 310029 1 Laboratory of Fruit Molecular Physiology and Biotechnology/ The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Biotechnology, Huajiachi Campus, Hangzhou 310029,China;2.College of Life Sciences, Zhejiang University, Huajiachi Campus, Hangzhou 310029,China
基金项目:国家重点基础研究发展规划项目(973项目,G2000046806),国家自然科学基金(30270917),浙江省自然科学基金(ZD0004)重点项目资助~~
摘    要:根据多年生植物组织富含多酚、多糖的具体特性,对现有的DNA提取方法进行了改进。通过增加提取缓冲液中b-巯基乙醇用量,简化氯仿/异戊醇抽提液步骤,改用经-20℃预冷异丙醇沉淀DNA等,对CTAB法加以改进。改进后方法具有以下优点:(1)获得的DNA质量良好,提取过程无明显的DNA降解,基本上排除了多酚物质的干扰;(2)用获得的DNA进行Southern杂交,可得到理想的杂交信号,可满足相关的分子研究要求;(3)操作简便。Abstract It is a difficult problem to isolate high quality DNA from plants containing a high contents of polyphenolics and polysaccharose, such as Actinidia plant. The protocol described in this paper is a modified CTAB (hexadecyltrimethylammonium bromide) method. High quality genomic DNA can be isolated from Actinidia plant using the improved method. The DNA is good enough for Southern blot and other uses in DNA research. The protocol is also efficient for quick and macro-DNA extraction.

关 键 词:DNA  猕猴桃  Southern杂交Key  words  Actinidia  提取  
文章编号:0253-9772(2004)04-0529-03
修稿时间:2003年4月7日

An Efficient Macro-method of Genomic DNA Isolation from Actinidia chinensis Leaves
CHEN Kun-Song,LI Fang,XU Chang-Jie,ZHANG Shang-Long,FU Cheng-Xin.An Efficient Macro-method of Genomic DNA Isolation from Actinidia chinensis Leaves[J].Hereditas,2004,26(4):529-531.
Authors:CHEN Kun-Song  LI Fang  XU Chang-Jie  ZHANG Shang-Long  FU Cheng-Xin
Institution:Laboratory of Fruit Molecular Physiology and Biotechnology/The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Biotechnology, Huajiachi Campus, Hangzhou 310 029, China. akun@zju.edu.cn
Abstract:It is a difficult problem to isolate high quality DNA from plants containing a high contents of polyphenolics and polysaccharides, such as Actinidia plant. The protocol described in this paper is a modified CTAB (hexadecyltrimethylammonium bromide) method. High quality genomic DNA can be isolated from Actinidia plant using the improved method. The DNA is good enough for Southern blot and other uses in DNA research. The protocol is also efficient for quick and macro-DNA extraction.
Keywords:Actinidia  genomic DNA  isolation  Southern blot
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