| 大豆曲茎性状的遗传分析和RAPD标记研究 |
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| 引用本文： | 黄方,赵团结,孟庆长,章元明,盖钧镒,喻德跃.大豆曲茎性状的遗传分析和RAPD标记研究[J].遗传,2006,28(1):50-56. |
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| 作者姓名： | 黄方 赵团结 孟庆长 章元明 盖钧镒 喻德跃 |
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| 作者单位： | （南京农业大学国家大豆改良中心，作物遗传与种质创新国家重点实验室,南京 210095） |
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| 基金项目： | 国家重点基础研究发展计划(973计划)，教育部科学技术研究项目 |
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| 摘 要： | 曲茎遗传材料NG94-156与遗传背景不同的3个正常茎品种杂交,获得1个F2群体和2个重组自交家系(F7:8)。后代分离分析的结果表明，NG94-156的曲茎性状受两对隐性重叠基因控制。利用4个亲本和1个重组自交家系筛选260个RAPD随机引物，其中有1个引物S-506扩增出的多态性条带有较好的重复性。经过连锁分析，RAPD标记S-5061600与控制曲茎的基因的遗传距离为6.94 cM。
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| 关 键 词： | 大豆 曲茎 重组自交家系 F2群体 遗传分析 |
| 文章编号： | 0253-9772(2006)01-0050-07 |
| 收稿时间： | 2005-02-23 |
| 修稿时间： | 2005-06-01 |
Genetic Analysis and RAPD Marker of the Genes for Brachytic Stem Trait in Soybean |
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| HUANG Fang,ZHAO Tuan-Jie,MENG Qing-Chang,ZHANG Yuan-Ming,GAI Jun-Yi,YU De-Yue.Genetic Analysis and RAPD Marker of the Genes for Brachytic Stem Trait in Soybean[J].Hereditas,2006,28(1):50-56. |
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| Authors: | HUANG Fang ZHAO Tuan-Jie MENG Qing-Chang ZHANG Yuan-Ming GAI Jun-Yi YU De-Yue |
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| Institution: | (National Center for Soybean Improvement, and National Key Laboratory for Crop Genetics and Germplasm Enhancement，Nanjing Agricultural University，Nanjing 210095, China) |
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| Abstract: | Three crosses between NG94-156 (brachytic stem) and three varieties (normal stem) were made, and F2 segregative population and two recombined inbred line populations(F(7:8)) were obtained. Genetic analysis indicated that the brachytic stem of NG94-156 was controlled by two duplicate recessive genes. In searching for RAPD marker linked to the genes controlling brachytic stem, 260 RAPD primers were applied to screen four parents of three combinations and RIL. Polymorphic bands revealed by the primer S-506 exhibited the best repeatability among all primers. Linkage analysis indicated the genetic distance between S-506(1600) and brachytic stem gene was 6.94 cM. |
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| Keywords: | Soybean brachytic stem F2 population RIL populations genetic analysis RAPD marker |
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