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波尔山羊杂交后代及其亲本随机扩增多态DNA研究
引用本文:李祥龙,田庆义,马国强,刘金福,冯敏山,牛一兵,孙乃权,马广星,郭润民.波尔山羊杂交后代及其亲本随机扩增多态DNA研究[J].遗传,2000,22(2):75-77.
作者姓名:李祥龙  田庆义  马国强  刘金福  冯敏山  牛一兵  孙乃权  马广星  郭润民
作者单位:1.河北职业技术师范学院动物科学系,昌黎 066600 2.河北省昌黎县畜牧局,昌黎 066600 1.Dept.of Animal Science,Hebei Vocation_technical Teachers College,Changli 066600 2.Animal Husbandry Bureau of Changli County, Hebei Province,Changli 066600,China
基金项目:河北省科委博士基金资助项目!(课题编号 :98540411D)
摘    要:利用随机扩增多态DNA技术研究了波尔山羊、唐山奶山羊、青龙本地山羊以及波尔山羊与这两个山羊群体杂交后代共计128个山羊个体的随机扩增多态DNA。结果表明:(1)总群体平均遗传多样性指数(Hsp)为0.6974,群体遗传分化指数为0.9706,山羊群体间平均遗传距离指数(0.1314~0.2052)明显大于群体内的相应值(0.0582~0.1440),上述结果说明,所研究山羊群体不仅具有较为丰富的遗传多样性,而且其核基因组遗传变异主要存在于群体间。(2)山羊群体间的分子聚类关系与各群体间的亲缘关系基本一致。 Abstract:The random amplified polymorphic DNA(RAPD)of 128 individuals was studied,which were from Boer goat, Tangshan dairy goat,Qinglong native goat and their hybrids crossbred with Boer goat.The average index of genetic polymorphism for whole population(Hsp)and the index of genetic differentiation were 0.6974 and 0.9706,respectively.The average index of genetic distance between populations(0.1314~0.2052)was significantly higher than that within populations(0.0582~0.1440).All of these indicated that the genetic polymorphism was not only abundant,but also the genetic variation was mainly existed between goat populations.The molecular dendrogram among goat populations was in accord with their genetic relationship.

关 键 词:波尔山羊  杂交改良  随机扩增多态DNA  遗传多样性  亲缘关系  Key  words  
修稿时间:1999-03-11

Studies of Random Amplified Polymorphic DNA(RAPD)of Different Goat Populations Crossbred by Boer Goat
LI Xiang-long,TIAN Qing-yi,MA Guo-qiang,LIU Jin-fu,FENG Min-shan,NIU Yi-bing,SUN Nai-quan,MA Guang-xing,GUO Run-min.Studies of Random Amplified Polymorphic DNA(RAPD)of Different Goat Populations Crossbred by Boer Goat[J].Hereditas,2000,22(2):75-77.
Authors:LI Xiang-long  TIAN Qing-yi  MA Guo-qiang  LIU Jin-fu  FENG Min-shan  NIU Yi-bing  SUN Nai-quan  MA Guang-xing  GUO Run-min
Abstract:The random amplified polymorphic DNA(RAPDof 128 individuals was studied,which were from Boer goatTangshan dairy goat,Qinglong native goat and their hybrids crossbred with Boer goat.The average index of genetic polymorphism for whole populationHspand the index of genetic differentiation were 0.6974 and 0.9706,respectively.The average index of genetic distance between populations0.13140.2052was significantly higher than that within populations0.05820.1440.All of these indicated that the genetic polymorphism was not only abundantbut also the genetic variation was mainly existed between goat populations.The molecular dendrogram among goat populations was in accord with their genetic relationship.
Keywords:boer goat  crossbreeding  RAPD  genetic diversity  genetic relationship
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