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用天花粉蛋白基因转化小麦获得转基因植株
引用本文:徐琼芳,李连城,马有志,叶兴国,杜丽璞,辛志勇,张增艳,李晓兵,邵鹏柱.用天花粉蛋白基因转化小麦获得转基因植株[J].遗传,2001,23(2):135-137.
作者姓名:徐琼芳  李连城  马有志  叶兴国  杜丽璞  辛志勇  张增艳  李晓兵  邵鹏柱
作者单位:1. 中国农业科学院作物育种栽培研究所,
2. 中国科学院遗传研究所
3. 香港中文大学生物系
基金项目:国家自然科学基金(39770458)资助
摘    要:取普通小麦品种京411未成熟胚诱导愈伤组织,10天左右,对820个胚性愈伤组织用含有35S启动子的天花粉蛋白(trichosanthin,TCS)基因轰击。2周后,将这些被轰击的愈伤组织转到含潮霉素50mg/L的筛选培养基上,经分化和生根,获得了33棵再生植株,经接饲毒蚜虫抗病性鉴定和PCR,Southern杂交分析,从中筛选出4株含有编码TCS的转基因小麦植株,转化频率为0.49%。

关 键 词:转基因小麦  TCS基因  转化  基因枪  天花粉蛋白基因
文章编号:0253-9772(2001)02-0135-03
修稿时间:2000年5月23日

Wheat Transformation with Trichosantin Gene by Biolistic Particle
XU Qiong-Fang,LI Lian-cheng,MA You-Zhi,YE Xing-guo,DU Li-Pu,XIN Zhi-yong,ZHANG Zeng-Yan,LI Xiaobing,Pang-Chui Shaw.Wheat Transformation with Trichosantin Gene by Biolistic Particle[J].Hereditas,2001,23(2):135-137.
Authors:XU Qiong-Fang  LI Lian-cheng  MA You-Zhi  YE Xing-guo  DU Li-Pu  XIN Zhi-yong  ZHANG Zeng-Yan  LI Xiaobing  Pang-Chui Shaw
Institution:XU Qiong-fang1,LI Lian-cheng1,MA You-zhi1,YE Xing-guo1 LI Xiaobing2,DU Li-pu1,XIN Zhi-yong1,ZHANG Zeng-yan1,Pang-Chui Shaw3
Abstract:Wheat immature embryo calli of 10 days culture from Jing 411,a common wheat variety, Triticum aestivum, were used as the receptor of Trichosanthin gene droven by 35S promotor to be bombardmented.Two weeks after b ombardment, 820 embryonic calli were transferred onto selection medium containin g 50mg/L hygromycin,and 33 plantlets with healthy roots were regenerated finaly from the calli. By the barley yellow dwarf virus resistance test of inoculating the wheat aphids carrying the virus on the plants, PCR analysis, and Southern b lotting analysis, 4 plants were identified to be the transgenic wheats, containi ng the alien DNA that ecodes TCS gene. The transformed efficiency was around 0.1 2 percent.
Keywords:transgenic wheat  trichosantin gene  transformation by biolistic
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