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利用小麦微卫星引物建立簇毛麦染色体组特异性标记
引用本文:刘成,杨足君,冯娟,周建平,迟世华,任正隆.利用小麦微卫星引物建立簇毛麦染色体组特异性标记[J].遗传,2006,28(12):1573-1579.
作者姓名:刘成  杨足君  冯娟  周建平  迟世华  任正隆
作者单位:电子科技大学生命科学与技术学院,成都610054
摘    要:选位于普通小麦1A-7A、1B-7B、1D-7D染色体上的102对微卫星引物对多年生簇毛麦、二倍体簇毛麦、小麦-簇毛麦双二倍体与后代和普通小麦中国春、R25、R111、MY11进行了PCR扩增, 发现引物对Xgwm301可以在含簇毛麦染色体的材料中扩出一条长415 bp的特异片段(命名为Xgwm301/415), 而所有供试小麦均未扩出此片段。进而用一套中国春-二倍体簇毛麦附加系来进行扩增, 发现1V-7V染色体均可以扩出该片段, 说明该片段为簇毛麦1V-7V染色体所共有。因此, Xgwm301/415是簇毛麦染色体组上的一个特异片段, 可以用来快速跟踪检测导入到普通小麦背景中的簇毛麦染色体。

关 键 词:簇毛麦  SSR  基因组特异性标记
文章编号:0253-9772(2006)12-1573-07
收稿时间:2005-11-09
修稿时间:2006-03-17

Development of Dasypyrum Genome Specific Marker by Using Wheat Microsatellites
LIU Cheng,YANG Zu-Jun,FENG Juan,ZHOU Jian-Ping,CHI Shi-Hua,REN Zheng-Long.Development of Dasypyrum Genome Specific Marker by Using Wheat Microsatellites[J].Hereditas,2006,28(12):1573-1579.
Authors:LIU Cheng  YANG Zu-Jun  FENG Juan  ZHOU Jian-Ping  CHI Shi-Hua  REN Zheng-Long
Institution:School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, China
Abstract:One hundred and two SSR primer pairs, distributed in chromosome 1A to 7A, 1B to 7B, 1D to 7D of Triticum aestivum, were investigated on Dasypyrum breviaristatum, D.villosum, wheat-Dasypyrum amphiploids and its derivatives, with the control of common wheat Chinese Spring and elite wheat cultivars. A specific polymorphic DNA fragment of about 400 bp ( 415 bp-long by sequenced, named Xgwm301/415) amplified by primer pair Xgwm301 was obtained in all lines containing Dasypyrum chromosomes, but there were not the case in the tested common wheat. Furthermore, PCR analysis was performed on a set of T. aestivum-Dasypyrum addition, the result showed that all the seven pairs of villosum chromosomes contain Xgwm301/415. Therefore, Xgwm301/415 is a genome -specific polymorphic DNA segment for genera of Dasypyrum, and it could be used as a molecular marker for detection of chromosomes of Dasypyrum in wheat.
Keywords:SSR
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