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优质细毛羊羊毛细度的候选基因分析
引用本文:刘桂芬,田可川,张恩平,黄锡霞,张艳花.优质细毛羊羊毛细度的候选基因分析[J].遗传,2007,29(1):70-74.
作者姓名:刘桂芬  田可川  张恩平  黄锡霞  张艳花
作者单位:1. 中国农业大学动物科技学院,北京,100094
2. 新疆畜牧科学院畜牧研究所,乌鲁木齐,830000
3. 西北农林科技大学动物科技学院,陕西杨凌,712100
4. 新疆农业大学动物科学学院,乌鲁木齐,830052
基金项目:国家科技攻关计划,国家自然科学基金,新疆维吾尔自治区高新技术项目
摘    要:采用PCR-SSCP的分子标记技术, 选择编码羊毛纤维组成蛋白基因中的KAP1.1、KAP1.3的部分序列、KAP6.1的外显子区作为候选基因, 通过对其多态性的研究, 探索将该基因作为候选基因来间接选择羊毛细度性状的可行性。得出其中在角蛋白辅助蛋白的多基因家族中的高硫蛋白辅助蛋白基因(KAP1.1、KAP1.3)中, 位点W08667与羊毛细度有显著的相关性(P<0.05)。在甘氨酸酪氨酸角蛋白辅助蛋白中, 外显子位点W06933的AA基因型和BB基因型与羊毛细度之间有显著的相关性(P<0.05)。

关 键 词:优质细毛羊  羊毛细度  候选基因  PCR-SSCP
收稿时间:2006-04-12
修稿时间:04 12 2006 12:00AM

Candidate gene analysis of high quality merino sheep
LIU Gui-Fen,TIAN Ke-Chuan,ZHANG En-Ping,HUANG Xi-Xia,ZHANG Yan-Hua.Candidate gene analysis of high quality merino sheep[J].Hereditas,2007,29(1):70-74.
Authors:LIU Gui-Fen  TIAN Ke-Chuan  ZHANG En-Ping  HUANG Xi-Xia  ZHANG Yan-Hua
Institution:1. College of Animal Science and Technology, Chinese Agricultural University, Beifing 100094, China; 2. Xinfiang Academy of Animal Science, Urumqi, Xinjiang 830000, China; 3. College of Animal Science and Technology, Northwest Sc-Tech University of Agriculture and Forestry, Yangling, Shaanxi 712100, China; 4. College of Animal Science and Technology, Xinjiang Agricultural University, Urumqi, Xinfiang 830052, China
Abstract:Partial sequences of wool fiber constituent genes KAP1.1 and KAP1.3 and the exonic sequence of the KAP6.1 gene were chosen for polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) analysis to assess their ability as candidate genes during indirect selection for fine wool traits. Results show that locus W08667 in the genes (KAP1.1, KAP1.3) which code the high sulfur protein associated-protein of keratin associated-protein family is significantly correlated with fine wool quality (P<0.05). Among the high-glycine-tyrosine keratin associated- protein, the AA and BB genotypes of W06933 are also significantly correlated with fine wool quality (P<0.05).
Keywords:PCR-SSCP
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