PCR扩增近交系大鼠微卫星位点DNA多态性的研究

本实验选取大鼠7条染色体上的微卫星位点合成了10对引物，利用聚合酶链反应（PCR）扩增技术对国内北京和哈尔滨等4家单位提供伯6个品系（SHR、SHRSP、LEW、RCS、WKY和F344）的8个近交系大鼠群体进行了DNA多态性分析的研究。结果表明：9个微卫星位点具有显多态性；不同品系个体之间具有多态性；同一群体不同个体之间除SHR（哈）的SMST位点和WKY（哈）的AGT位点出现一定的差异，其他均没有差异；不同地区同一品系的不同个体之间也存在一定的差异。该方法能有效地对近交系与杂交系、品系与品系、品系与亚系加以区分。因此，本实验为开展近交系大鼠遗传作图、基因定位和为实验动物的遗传背景监测提供可靠的信息，为大鼠遗传基因的研究提供了一个快速简例、特异准确的方法。

Rat Gene Polymorphism Using PCR-Analyzed Microsatellites

In the experiment,It were selected that 20 primers were assigned on 7 chromosomes of inbred rat.DNA polymorphism of 8 colonies from 6 inbred rat strains (SHR?SHRSP?WKY?LEW?RCS?F344) were studied in national Beijing and Harbin using PCR-analyzed microsatellites. The results indicated that there were remarkable polymorphism in 9 microsatellite loci;There is a polymorphism among the various rat strains;and no polymorphism in the same rat strains except SMST locus of SHR(Harbin)and AGT locus of WKY(Harbin)and there is a little difference among the same inbred rat strains in different areas.The method of PCR-analyzed microsatellites can be used for distinguishing between inbred and outbred ?different strains?strain and substrain.and it provides a lot of information for genetic mapping,gene location and heredity probe of the inbred rat strains,and a speedy?convenient method for genetic research of the inbred rat.