| 抗生素对大豆愈伤组织的诱导和生长的影响 |
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| 引用本文： | 王萍,.吴颖,季静,王罡,杨庆凯WANG Ping,WU Ying,JI JingI,WANG Gang,YANG Qing-kai.抗生素对大豆愈伤组织的诱导和生长的影响[J].遗传,2001,23(4):321-761. |
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| 作者姓名： | 王萍 .吴颖 季静 王罡 杨庆凯WANG Ping WU Ying JI JingI WANG Gang YANG Qing-kai |
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| 作者单位： | 1.解放军军需大学植物分子生物学实验室,长春 130062;
2.东北农业大学大豆研究所,哈尔滨 150030
1.Laboratory of Plant Molecule Biology,Quartermaster University,Changchun 130062,China;
2.The Soybean Institute,Northeast Agricultural University,Harbin 150030,China |
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| 摘 要： | 用红霉素、头孢唑啉钠、头孢拉定、头孢霉素(国产和进口)等5种抗生素对农杆菌LBA4404进行抑菌试验,以头孢霉素的抑菌效果最好.头孢霉素作为抑菌剂用于大豆遗传转化试验时,在下胚轴浓度以300mg/L,在子叶节以500mg/L为宜.大豆品种对卡那霉素的反应在出愈率上表现相似,在褐化率上表现有些不同.大豆不同外植体对卡那霉素的反应存在较大差异,以真叶反应最敏感,下胚轴反应最迟钝.在以卡那霉素作为抗性选择标记时,选择压力真叶和子叶节以50～100mg,L为好,下胚轴以100～200mg/L为宜。
Ahstract:The experiment of inhibiting Agrobacterium LBA4404 was undertaken with 5 antibiotics (the Erythronycin Base,Cefazolin Sodium,Cefradine,2 kinds of Cefotaximes).Among them,Cefotaxime showed the best effect.When Cefotaxime is used in transformation,the ideal concentration is 300mg/L in hypocotyl and 500mg/L in cotyledon node.The response of soybean varieties to Kanamycin is similar in induction of callus rate and is different in brown rate of callus.Differences of the response of soybean explants to Kanamycin were found.The young leaves are sensitive to Kanamycin,but hypoeotyl is not.The ideal selecting pressure of Kanamycin is 50- l00mg/L in young leaf and cotyledon node,andl00-200mg/L in hypocotyl when Kanamycin is used as selection marker.
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| 关 键 词： | 大豆(Glycine max L.) 抗生素 农杆菌 soybean 愈伤组织 Key words |
Effect of Antibiotics on Induction of Callus and Callus Growth in Soybean |
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| WANG Ping,WU Ying,JI JingI,WANG Gang,YANG Qing-kai.Effect of Antibiotics on Induction of Callus and Callus Growth in Soybean[J].Hereditas,2001,23(4):321-761. |
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| Authors: | WANG Ping WU Ying JI JingI WANG Gang YANG Qing-kai |
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