| 美洲黑杨抗黑斑病基因的RAPD标记筛选和连锁分析 |
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| 引用本文： | 张博,黄敏仁,诸葛强,韩正敏,尹佟明,潘惠新,朱立煌,邬荣领,王明庥.美洲黑杨抗黑斑病基因的RAPD标记筛选和连锁分析[J].遗传,2002,24(5):543-547. |
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| 作者姓名： | 张博 黄敏仁 诸葛强 韩正敏 尹佟明 潘惠新 朱立煌 邬荣领 王明庥 |
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| 作者单位： | 1.南京林业大学林木遗传和基因工程重点实验室，南京210037；
2.中国科学院遗传与发育生物学研究所植物生物技术重点实验室，北京100101；
3.美国佛罗里达大学统计系,佛罗里达32611 |
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| 基金项目： | 国家自然科学基金重点项目资助（No.39930140），International Foundation for Science（IFS,瑞典）Project（No.D2997-1） |
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| 摘 要： | 以美洲黑杨（Populus deltoides Bartr.cv.“Lux”(I-69/55)）为母本，欧美杨（P.euramericana cv.I-45）为父本得到的F1为材料，其中I-69对黑斑病表现为高度抗病，I-45为高度感病。利用分离群体混合分析（bulked segregrants analysis，BSA）技术建立两个DNA池（感病池和抗病池），筛选出了与抗黑斑病性状基因相连锁的RAPD标记:OPAI17―1550、OPAI13―900。利用选择性基因型分析法进行标记―抗黑斑病基因连锁分析，两标记与抗黑斑病基因遗传距离分别为29.9cM和37.4cM。
Identification of Markers Linked to Resistance Locus of Marssonina Leaf Spot in Poplars by Bulked Segregant Analysis(BSA)
ZHANG Bo1,HUANG Min-ren1，ZHUGE Qiang1，HAN Zheng-min1，YIN Tong-ming1，PAN Hui-xin1，ZHU Li-huang2，WU Rong-ling3,WANG ming-xiu１
1.The Key Laboratory of Forest Tree Genetic Engineering,Nanjing Forestry University,Nanjing,210037,China;
2.The Institute of Genetics and Developmental Biology,Chinese Academy of Science,Beijing 100101,China;
3.Department of Statistics,University of Florida,Gainesville,Florida 32611,USA
Abstract:DNA markers linked to resistance locus of Marssonina leaf spot in poplars were found by bulked segregant analysis(BSA).The bulks consisted of individual with a extreme phenotype taken from a population of 91 F1 clones,which is a progeny of Populus deltoides Bartr.cv.“Lux”(I-69/55)(Resistance) and P.euramericana cv.I-45(Susceptible).Out of 114 RAPD primers,four markers showed polymorphisms between the resistance-bulk and the susceptible-bulk.By using selective genotype linkage analysis,OPAI17-1550 and OPAI13-900 were found linked to the resistance locus.The genetic distances between the two markers and the resistance locus were 29.9cM and 37.4cM,respectively.
Key words：BSA; RAPD; selective genotype analysis; Marssonina leaf spot
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| 关 键 词： | BSA RAPD 选择性基因型分析法 杨树黑斑病 |
| 文章编号： | 0253-9772(2002)05-0543-05 |
| 修稿时间： | 2001年12月27 |
Identification of Markers Linked to Resistance Locus of Marssonina Leaf Spot in Poplars by Bulked Segregant Analysis(BSA) |
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| ZHANG Bo,HUANG Min-ren,ZHUGE Qiang,HAN Zheng-min,YIN Tong-ming,PAN Hui-xin,ZHU Li-huang,WU Rong-ling,WANG ming-xiu The Key Laboratory of Forest Tree Genetic Engineering,Nanjing Forestry University,Nanjing,China,The Institute of Genetic.Identification of Markers Linked to Resistance Locus of Marssonina Leaf Spot in Poplars by Bulked Segregant Analysis(BSA)[J].Hereditas,2002,24(5):543-547. |
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| Authors: | ZHANG Bo HUANG Min-ren ZHUGE Qiang HAN Zheng-min YIN Tong-ming PAN Hui-xin ZHU Li-huang WU Rong-ling WANG ming-xiu The Key Laboratory of Forest Tree Genetic Engineering Nanjing Forestry University Nanjing China The Institute of Genetic |
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| Institution: | The Key Laboratory of Forest Tree Genetic Engineering, Nanjing Forestry University, Nanjing,210037, China. zhangbo1978@hotmail.com |
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| Abstract: | DNA markers linked to resistance locus of Marssonina leaf spot in poplars were found by bulked segregant analysis (BS A). The bulks consisted of individual with a extreme phenotype taken from a population of 91 Fj clones, which is a progeny of Populus deltoides Bartr. cv. "Lux"(I-69/55) (Resistance) and P. euramericana cv. I-45(Susceptible). Out of 114 RAPD primers,four markers showed polymorphisms between the resistance-bulk and the susceptible-bulk. By using selective genotype linkage analysis, OP All 7 -1550 and OPAI13 - 900 were found linked to the resistance locus. The genetic distances between the two markers and the resistance locus were 29. 9cM and 37. 4cM,respectively. |
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| Keywords: | BSA RAPD selective genotype analysis Marssonina leaf spot |
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