我国主要地方绵羊品种随机扩增多态DNA研究

对蒙古羊、湖羊、滩羊、小尾寒羊、乌珠穆沁羊、藏绵羊、阿勒泰羊7个地方绵羊品种和无角陶赛特羊、德国美利奴羊、萨福克羊3个引入品种基因组DNA进行了RAPD分析。结果表明：（1）RAPD可作为一种有效的标记用于绵羊品种之间遗传亲缘关系的分析。（2）在所使用的43种随机引物中，有35种引物扩增出多态谱带，多态频率为66.24%，说明RAPD技术用于研究绵羊核DNA的遗传变异具有较高的检出率和灵敏度。（3）总群体平均遗传多样性指数（HSP)为0.9139，说明绵羊群体具有较为丰富的遗传多样性。（4）我国地方绵羊品种间的分子聚类关系与其所处的地理位置、考古学结果，以及细胞遗传学研究结果基本，引入品种间的分子聚类关系也与其育成史基本一致。

Studies of Random Amplified Polymorphic DNA(RAPD) of Main Indigenous Sheep Breeds in China

The genetic polymorphism and relationship of 7 indigenous sheep breeds of China and 3 imported sheep breeds were studied using random amplified polymorphic DNA (RAPD). The results indicated that the RAPD was an effective marker for the analysis of genetic relationship among sheep breeds. Among 43 arbitrary primers,35 were polymorphic. The percentage of polymorphic markers was 66. 24% , which indicated that the RAPD had higher effi-ciency of polymorphism detection and sensitivity in studying the genetic variation among sheep breeds. The average index of genetic polymorphism for whole population (Hsp) was 0. 9139, which showed that the genetic polymor-phism was abundant between sheep populations. The genetic relationship between different indigenous sheep breeds in China was in accord with their localities,the results from archeology and cytogenetics and the genetic relationship between imported sheep breeds was in accord with their breeding history.