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浙东白鹅催乳素基因表达特点
引用本文:褚晓红,徐宁迎,胡锦平,卢立志,陈维虎,王亚琴.浙东白鹅催乳素基因表达特点[J].遗传,2008,30(8):1021-1025.
作者姓名:褚晓红  徐宁迎  胡锦平  卢立志  陈维虎  王亚琴
作者单位:1. 浙江大学动物科学学院,杭州,310029;浙江省农业科学院畜牧兽医研究所,杭州,310021
2. 浙江大学动物科学学院,杭州,310029
3. 浙江省农业科学院畜牧兽医研究所,杭州,310021
4. 浙江省宁波市象山农林局,宁波,315700
基金项目:国家重点基础研究发展计划(973计划),浙江省科技计划
摘    要:克隆了浙东白鹅催乳素基因(Prolactin, PRL)的全序列, 并应用荧光定量PCR技术研究了浙东白鹅在产蛋期、就巢期和恢复期时催乳素基因在下丘脑、垂体和卵巢中的表达特点。结果表明, 浙东白鹅催乳素基因在就巢期、产蛋期和恢复期的表达量差异显著(P < 0.05), 在就巢期表达量最高, 产蛋期次之, 恢复期表达量最低。对不同组织PRL的表达量分析, 发现在垂体与卵巢中的表达量、卵巢与下丘脑的表达量均有极显著的差异(P < 0.01), 但在垂体与下丘脑中的表达量差异不显著(P > 0.05), 在垂体表达量最多, 其次是下丘脑, 卵巢中的表达量最低。因此, 浙东白鹅PRL基因在不同繁殖时期体内表达差异较大。

关 键 词:催乳素基因  浙东白鹅  荧光定量PCR  表达量
收稿时间:2007-11-30
修稿时间:2008-02-19

Expression characteristics of prolactin gene in Eastern Zhejiang white geese
CHU Xiao-Hong,XU Ning-Ying,HU Jin-Ping,LU Li-Zhi,CHEN Wei-Hu,WANG Ya-Qin.Expression characteristics of prolactin gene in Eastern Zhejiang white geese[J].Hereditas,2008,30(8):1021-1025.
Authors:CHU Xiao-Hong  XU Ning-Ying  HU Jin-Ping  LU Li-Zhi  CHEN Wei-Hu  WANG Ya-Qin
Institution:1. College of Animal Science, Zhejiang University, Hangzhou 310029, China; 2. Institute of Animal Science and Veterinary, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China E-mail: chuxh@yahoo.cn.
Abstract:This study was conducted to clone the prolactin gene (PRL) in Eastern Zhejiang White Geese and to investigate the PRL gene expression characteristics during egg-laying, out-of-lay and incubating periods by real time PCR. Comparisons were made respectively of concentration of prolactin mRNA in the hypothalamus, pituitary gland and ovary of the adult female geese at different reproductive periods. The result indicated that there were significant differences (P < 0.05) in PRL mRNA expression between different reproductive periods of the geese. The lowest level of PRL expression was found in out-of-lay geese, higher in the egg-laying geese, and the highest in incubating geese. Furthermore, the analysis of PRL expression in different tissues indicated that the highest levels of PRL was expressed in the pituitary gland, followed in hypothalamus, and the least in ovary of the geese. There were significant difference (P < 0.01) expression of PRL between the pituitary gland/hypothalamus and ovary of the geese, whereas no any difference was observed between the pituitary gland and hypothalamus (P > 0.05). In summary, the PRL mRNA expression had varianced in different reproductive periods of the geese.
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