| 用交错延伸剪接PCR技术扩增油菜花粉育性基因MS2Bnap全长cDNA序列 |
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| 引用本文： | 李德谋,罗小英,侯磊,裴炎,方卫国,杨光伟LI De-mou,LUO Xiao-ying,HOU Lei,PEI Yan,FANG Wei-guo,YANG Guang-wei.用交错延伸剪接PCR技术扩增油菜花粉育性基因MS2Bnap全长cDNA序列[J].遗传,2001,23(6):553-331. |
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| 作者姓名： | 李德谋 罗小英 侯磊 裴炎 方卫国 杨光伟LI De-mou LUO Xiao-ying HOU Lei PEI Yan FANG Wei-guo YANG Guang-wei |
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| 作者单位： | 西南农业大学生物技术中心,重庆,北碚,400716
Center for Biotechnology,SUTH west Agricultural University,Chongqing 400716,China |
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| 摘 要： | 交错延伸剪接PCR是一种用于分子进化研究的DNA改组技术.本实验利用其原理,将已获得的油菜花粉育性基因MS2Bnap有部分序列重叠的三段PCR产物作为模板进行扩增,获得了花粉育性基因MS2Bnap的全长cDNA序列。
Abstract:SOE by PCR is one of DNA shuffling technologies for molecular evolution engineering.Using the theory of SOE by PCR,the full-length cDNA sequence of pollen fertility gene MS2Bnap of Brasscia napus was amplified with three obtained fragments as templates which have part overlapping of MS2Bnap sequence.
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| 关 键 词： | 花粉育性基因MS2Bnap 交错延伸剪接PCR 全长cDNA序列 Key words Pollen fertility gene MS2Bnap splicing by overlap extension by PCR (SOE by PCR) |
Amplification of Pollen Fertility Gene MS2Bnap Full-length cDNA from Brassica napus Using SOE by PCR |
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| LI De-mou,LUO Xiao-ying,HOU Lei,PEI Yan,FANG Wei-guo,YANG Guang-wei.Amplification of Pollen Fertility Gene MS2Bnap Full-length cDNA from Brassica napus Using SOE by PCR[J].Hereditas,2001,23(6):553-331. |
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| Authors: | LI De-mou LUO Xiao-ying HOU Lei PEI Yan FANG Wei-guo YANG Guang-wei |
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