| 一步3’RACE快速构建鸡MnSOD全长cDNA克隆Rapid |
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| 引用本文： | 卜友泉,罗绪刚,刘彬,李素芬.一步3’RACE快速构建鸡MnSOD全长cDNA克隆Rapid[J].遗传,2004,26(4):519-521. |
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| 作者姓名： | 卜友泉 罗绪刚 刘彬 李素芬 |
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| 作者单位： | 中国农业科学院畜牧研究所，北京 100094 Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100094 China |
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| 基金项目： | 国家杰出青年科学基金(39925028)，北京市自然科学基金(6992022)，国家人事部留学回国基金(7)资助~~ |
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| 摘 要： | 本研究尝试将触减 PCR与3’ cDNA末端快速扩增（rapid amplification of cDNA ends，RACE）技术进行结合，仅用一条特异性引物和一条通用引物，成功地实现了从3’末端cDNA库对鸡含锰超氧化物歧化酶（manganese-containing superoxide dismutase，MnSOD）全长cDNA的一步3’RACE快速构建。与常规使用的末端PCR或亚克隆方法相比，该法具有快速、省时、经济和特异性好的优点。Abstract: RACE(rapid amplification of cDNA ends) is a popular technique to rapidly obtain the full-length cDNA. After obtaining the 3’cDNA and 5’cDNA fragments with a overlapped region by 3’RACE and 5’RACE, the full-length cDNA could be generated by end-to-end PCR or subcloning. In this study, 3’RACE combined with touch-down PCR was successfully used for the rapid construction of full-length MnSOD cDNA of chickens. Compared with the conventional end-to-end PCR or subcloning, this method, called one-step 3’RACE, is fast, economical and highly specific. It especially fits the rapid construction of full-length cDNA by RACE method.
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| 关 键 词： | RACE 触减PCR MnSOD RACE cDNA Key words |
| 文章编号： | 0253-9772(2004)04-0519-03 |
| 修稿时间： | 2003年5月26日 |
Rapid Construction of Full-length MnSOD cDNA of Chickens by One-step 3′RACE |
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| BU You-Quan,LUO Xu-Gang,LIU Bin,LI Su-Fen.Rapid Construction of Full-length MnSOD cDNA of Chickens by One-step 3′RACE[J].Hereditas,2004,26(4):519-521. |
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| Authors: | BU You-Quan LUO Xu-Gang LIU Bin LI Su-Fen |
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| Institution: | Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100 094, China. |
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| Abstract: | RACE (rapid amplification of cDNA ends) is a popular technique to rapidly obtain the full-length cDNA. After obtaining the 3' cDNA and 5' cDNA fragments with a overlapped region by 3' RACE and 5' RACE, the full-length cDNA could be generated by end-to-end PCR or subcloning. In this study, 3' RACE combined with touch-down PCR was successfully used for the rapid construction of full-length MnSOD cDNA of chickens. Compared with the conventional end-to-end PCR or subcloning, this method, called one-step 3' RACE, is fast, economical and highly specific. It especially fits the rapid construction of full-length cDNA by RACE method. |
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| Keywords: | RACE touch-down PCR MnSOD Cdna |
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