| 雄性不育嵌合基因的构建及番茄转化研究 |
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| 引用本文： | 张宏,王波,薛爱群,曹俊,李宝健,谭兆平,黄伟如ZHANG Hong,WANG Bo,XUE Ai-qun,CAO Jun,LI Bao-jian,TAN Zhao-ping,HUANG Wei-ru.雄性不育嵌合基因的构建及番茄转化研究[J].遗传,1998,20(3):5-7. |
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| 作者姓名： | 张宏 王波 薛爱群 曹俊 李宝健 谭兆平 黄伟如ZHANG Hong WANG Bo XUE Ai-qun CAO Jun LI Bao-jian TAN Zhao-ping HUANG Wei-ru |
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| 作者单位： | 1.广州中山大学生物工程研究中心,广州 510275
2.广州市蔬菜科学研究所，广州 510315
1.Biotechnology Research Center,Zhongshan University,Guangzhou 510275
2.Guangzhou Vegetable Scientific Research Institute,Guangzhou 510315 |
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| 基金项目： | 国家自然科学基金,广东省自然科学基金 |
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| 摘 要： | 用从烟草里克隆的TA29启动子分离自Bacillus amyloliquefaciens的核糖核酸酶基因barnase,构建成雄性不育嵌合基因。再用农杆菌介导法转化番茄子叶,获得了具有雄性不育特征的转基因植株。
Abstract:The utilization of heterosis based on male sterility has great significance in raising crop yeld and improving quality.A tapetum-specific promoter TA29 from tobacco young leaves,and a Rnase gene-barnase from Bacillus amyloliquefaciens have been cloned for the construction of male sterile chimeric gene.Transgenic tomato plants with male sterile property have been obtained after transformation with this gene.
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| 关 键 词： | 雄性不育 转基因 Male sterility 番茄 Key words Transgenic TH01 TPOX DP CSF1PO 中国汉族 STR |
The Constuction and Tomato Tranformation of Male SterileChimeric Gene |
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| ZHANG Hong,WANG Bo,XUE Ai-qun,CAO Jun,LI Bao-jian,TAN Zhao-ping,HUANG Wei-ru.The Constuction and Tomato Tranformation of Male SterileChimeric Gene[J].Hereditas,1998,20(3):5-7. |
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| Authors: | ZHANG Hong WANG Bo XUE Ai-qun CAO Jun LI Bao-jian TAN Zhao-ping HUANG Wei-ru |
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| Abstract: | |
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| Keywords: | Male sterility Transgenic Tomato |
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