| 基因枪转移Bar基因获得抗除草剂(Basta)的糜子蔗克隆再生植株 |
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| 引用本文： | 母秋华,原亚萍,贾玉峰,王金余,田文忠,何锶洁,李良材MU Qiu-hua,YUAN Ya-ping,JIA Yu-feng,WANG Jin-yu,TIAN Wen-zhong,HE Si-jie,LI Liang-cai.基因枪转移Bar基因获得抗除草剂(Basta)的糜子蔗克隆再生植株[J].遗传,1998,20(3):23-26. |
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| 作者姓名： | 母秋华 原亚萍 贾玉峰 王金余 田文忠 何锶洁 李良材MU Qiu-hua YUAN Ya-ping JIA Yu-feng WANG Jin-yu TIAN Wen-zhong HE Si-jie LI Liang-cai |
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| 作者单位： | 1.解放军农牧大学植物细胞工程研究室, 长春 130062
2.中国科学院遗传研究所，北京 100101
1.Plant Cell Engineering Lab.,University of Agricultural and Animal Science of PLA,Changchun 130062
2.Institute of Genetics,Chinese Academy of Sciences,Beijing 100101 |
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| 摘 要： | 用糜子蔗(sorghum×sugarcane)×millet sorghum茎尖愈伤组织为受体,通过基因枪轰击将Bar基因转入糜子蔗细胞;用除草剂Basta含量分别为1、2.5、5、10mg/L的8114诱导与分化培养基上选择抗性愈伤组织;在Bast a含量为1与1.5mg/L筛选培养基上,选择出3个快速增殖并大量再生绿苗的体细胞无性系(Clone),如BarC1、BarC2、BarC3;BarC1、C2再生植株用Basta含量为1、2.5-5mg/L的溶液进行抗性检测,证明这些小植株具有极强的抗性。
Abstract:The Bar genes were introduced into〔(sorghum×sugarcane) ×millet sorghum〕callus cells using particle bombardment.The callus were cultured on the 8114 media with Basta,the Bastas concentration is 1,2.5 and 5,10mg/L.3 Clones with regenerated capacity were screened out on the media with Basta 1,2.5mg/L,for example BarC1,BarC2,BarC3.The regenerated plant from BarC1、BarC2 were cultured on the solution with Basta 1.5,2.5,and 5mg/L,high resistance were obtained in these regenerated plants.
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| 关 键 词： | Bar genes transformation (Sorhum×Sugarcane) ×millet sorghum 植株再生 Key words Bar基因转移 糜子蔗 广亲和基因 不育性 遗传分析 水稻(Oryza sativa L.) 籼粳杂交 |
Regenerated Plants Resistanted to Basta were Obtained ThroughIntroduction of Bar Genes into [(Sorghum*Sugarcane)*Mi lletSorghum]Using Particle Bombardment |
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| MU Qiu-hua,YUAN Ya-ping,JIA Yu-feng,WANG Jin-yu,TIAN Wen-zhong,HE Si-jie,LI Liang-cai.Regenerated Plants Resistanted to Basta were Obtained ThroughIntroduction of Bar Genes into [(Sorghum*Sugarcane)*Mi lletSorghum]Using Particle Bombardment[J].Hereditas,1998,20(3):23-26. |
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| Authors: | MU Qiu-hua YUAN Ya-ping JIA Yu-feng WANG Jin-yu TIAN Wen-zhong HE Si-jie LI Liang-cai |
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| Abstract: | |
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| Keywords: | Sterility Oryza sativa Wide compatibility gene indica japonica cross Genetic analysis |
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