| 利用农杆菌系统将超甜定基因导入烟草 |
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| 引用本文： | 刘秋云,贺竹梅,曹俊,李宝健LIU Qiu-Yun,HE Zhu-Mei,CAO Jun,LI Bao-Jian.利用农杆菌系统将超甜定基因导入烟草[J].遗传,1998,20(1):24-27. |
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| 作者姓名： | 刘秋云 贺竹梅 曹俊 李宝健LIU Qiu-Yun HE Zhu-Mei CAO Jun LI Bao-Jian |
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| 作者单位： | 中山大学生物工程研究中心, 广州 510275
Biotechnology Research Center,Zhongshan University,Guangzhou 510275 |
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| 基金项目： | 广东省自然科学基金,国家教委博士后基金 |
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| 摘 要： | 由pUR528构建中间载体pEHT9,再构建超甜定植物表达载体pBIT7。通过直接转化法将pBIT7导入农杆菌,然后利用农杆菌系统转化烟草。经PCR、PC R-S outhern和Southern杂交证实,超甜定基因已整合到烟草基因组中。
Abstract:pEHT9 was constructed from pUR528,and used for the construction of plant thaumatin expression vector pBIT7.Then pBIT7 was introduced to Agrobacterium through direct transformation.Subsequently tobacco was transformed through Agrobacterium-mediated system.It has been confirmed that thaumatin gene has integrated into the genome of transgenic tobacco by PCR,PCR-Southern and Southern blot analyses.
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| 关 键 词： | 超甜定 烟草 农杆菌 转化 Key words Thaumatin 栽培稻 普通野生稻 RFLP 线粒体DNA |
Introduction of Thaumatin Gene into Tobacco through Agrobacterium -mediated System |
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| LIU Qiuyun,HE Zhumei,CAO Jun,LI Baojian.Introduction of Thaumatin Gene into Tobacco through Agrobacterium -mediated System[J].Hereditas,1998,20(1):24-27. |
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| Authors: | LIU Qiuyun HE Zhumei CAO Jun LI Baojian |
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| Abstract: | pEHT9 was constructed from pUR528, and used for the construction of plant thaumatin expression vector pBIT7. Then pBIT7 was introduced to Agrobacterium through direct transformation. Subsequently tobacco was transformed through Agrobacterium -mediated system. It has been confirmed that thaumatin gene has integrated into the genome of transgenic tobacco by PCR, PCR-Southern and Southern blot analyses. |
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| Keywords: | Thaumatin Tobacco Agrobacterium tumefaciens Transformation |
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