| 应用RNAi技术培育抗TMV病毒转基因烟草 |
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| 引用本文： | 颜培强,白先权,万秀清,郭兆奎,李丽杰,公海英,储成才.应用RNAi技术培育抗TMV病毒转基因烟草[J].遗传,2007,29(8):1018-1022. |
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| 作者姓名： | 颜培强 白先权 万秀清 郭兆奎 李丽杰 公海英 储成才 |
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| 作者单位： | 1. 黑龙江省烟草科学研究所,牡丹江,157011
2. 中国科学院遗传与发育生物学研究所,北京,100101 |
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| 基金项目： | 国家烟草专卖局资助项目 |
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| 摘 要： | 利用烟草花叶病毒(TMV)外壳蛋白基因构建RNAi干涉载体, 通过叶盘法转化至烟草K326 和龙江911两个栽培品种。对转基因株系的荧光定量PCR分析表明, 不同转基因株系的病毒RNA靶序列都得到一定程度的降解, 抗病性鉴定结果证实, 转基因K326和龙江911两个栽培品种的转基因材料分别有83％和90％转基因株系对TMV呈现免疫级抗性。
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| 关 键 词： | RNAi 转基因烟草 TMV |
| 文章编号： | 10.1360/yc-007-1018 |
| 收稿时间： | 2006-12-31 |
| 修稿时间： | 2006-12-31 |
Expression of TMV coat protein gene RNAi in transgenic tobacco plants confer immunity to tobacco mosaic virus infection |
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| YAN Pei-Qiang,BAI Xian-Quan,WAN Xiu-Qing,GUO Zhao-Kui,LI Li-Jie,GONG Hai-Ying,CHU Cheng-Cai.Expression of TMV coat protein gene RNAi in transgenic tobacco plants confer immunity to tobacco mosaic virus infection[J].Hereditas,2007,29(8):1018-1022. |
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| Authors: | YAN Pei-Qiang BAI Xian-Quan WAN Xiu-Qing GUO Zhao-Kui LI Li-Jie GONG Hai-Ying CHU Cheng-Cai |
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| Institution: | 1. Heilongjiang Tobacco Research Institute, Mudanfiang 157011, China; 2. Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beifing 100101, China |
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| Abstract: | RNAi technique has been proved as a powerful tool for plant breeding. In this paper,the coat protein of tobacco mosaic virus (TMV) was used for constructing the RNAi interference vector. The tobacco varieties K326 and Longjiang 911 were transformed via Agrobacterium tumefaciens-mediated transformation,and transgenic plants were generated. The expression analysis with real-time PCR indicated that TMV RNA had been degraded varied in different transgenic lines. Field assay revealed that 83% and 90 % transgenic plants showed immunity resistance to TMV in K326 and Longjiang 911 respectively. |
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| Keywords: | RNAi TMV |
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