培养基成分对东北红豆杉细胞生长和紫杉醇产量的影响

在东北红豆杉(taxuscuspidate)细胞培养物的基本培养基中加入30mg@L-12.4-D,可以提高细胞培养物的生长量,其相对最高生长量可以达到85.999mgFW/gFW@d;培养基中加入活性炭粉末lg@L-1,可以克服植物细胞生长过程中的褐化问题;适当种类及含量的生长调节剂的加入:6-BA0.5mg@L-1+KT2mg@L-1,可以使植物细胞合成和积累相对较多的紫杉醇(Taxol)。 Abstract:The growth quantity of Taxus Cuspidate's cell culture materials can be increased and the highest quantity will amount to 85.999 mgFW/g·FW·d·if We put 2,4-D (30rog·L-1 )into the basic cuhure medium of it; The difficulty of foxiness during the plants growing process can be overcome if we add acive carbon powder(lgL-1)into the culture medium; The plant cell can synthetize and gather more Taxol comparatively with the addition kf proper and amount of growth regulators:6-BA(0.5mg·L-1) + KT2.

The Relation between the Cell Growth,the Volume of Taxus Cuspidate and the Composition of its Cell Culture Medium