| 黄颡鱼染色体的C─带、Ag—NORs、荧光带和复制带显带的研究 |
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| 引用本文： | 耿德贵,张大生,程伟,邹寿昌,陈刚.黄颡鱼染色体的C─带、Ag—NORs、荧光带和复制带显带的研究[J].遗传,1999(2). |
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| 作者姓名： | 耿德贵 张大生 程伟 邹寿昌 陈刚 |
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| 作者单位： | 徐州师范大学生物系!徐州，221009，徐州师范大学生物系!徐州，221009，徐州师范大学生物系!徐州，221009，徐州师范大学生物系!徐州，221009，徐州师范大学生物系!江苏省阜宁县罗桥中学，徐州，221009 |
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| 摘 要： | 采用Giemas染色、C─带、Ag—NORs、荧光染色和复制带显带的技术对黄颡鱼染色体进行了研究。结果表明，黄颡鱼只有部分的染色体呈现阳性C─带，可分为三类，其中NORs区是染色最深、染色面积最大的区域，为深染居间C─带。其Ag－NORs位于m5q末端。CMA3染色显示NORs区呈现出明亮的荧光。中复制染色体上着丝粒区、端粒区和居间区浅染。发现核仁缢痕、深染居间C─带、Ag—NORs、CMA3明亮区和中复制带浅染NORs区位置基本一致，C─带阳性区和中复制带浅染区具有对应性。
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| 关 键 词： | 黄颡色 C─带 Ag—NORs 荧光染色 复制带 |
Chromosome Studies in Pelteobagrus fulvidraco by C-banding,Ag-NORs, Fluorochrome Staining and Replication Banding |
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| GENG De-Gui, ZHANG Da-Sheng, CHENG Wei, ZOU Shou-Chang, CHEN Gang.Chromosome Studies in Pelteobagrus fulvidraco by C-banding,Ag-NORs, Fluorochrome Staining and Replication Banding[J].Hereditas,1999(2). |
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| Authors: | GENG De-Gui ZHANG Da-Sheng CHENG Wei ZOU Shou-Chang CHEN Gang |
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| Institution: | Department of Biology. Xuzhou Normal University of Jiangsu Province. Xuzhou 221009 |
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| Abstract: | Metaphase chromosomes of Pelteobagrus fulvidraco were analyzed by means of Giemsa staining. C-banding, Ag-NORs, fluorochrome staining and replication banding. Only parts of chromosomesshowed C-band positive, the C-band heterochromatin was located in three regions. The NORs-bearingchromosomes had the largest and strongest C-bands. The long arms of chromosome pair 5 were the regions showing positive labeling with Ag-staining. Fluorochrome CMA3 positively stained the NORs. Inmid-period, negative replication bands were located in the centromere. the terminal and interstitial regionsof the chromosomes. The distributions of secondary constri-ctions, positive C-bands, Ag-NORs, positivefluorescence bands and negative replication bands of mid-period were coincident. |
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| Keywords: | Pelleobagrus fulvidraco C-banding Ag-NORs Fluorochrome staining Replication banding |
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