| 中国海南岛三个黎族支系DYS287、DYS19的多态性研究 |
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| 引用本文： | 李冬娜,应大君,区采莹,陈路,符生苗,谢协驹,陈峰.中国海南岛三个黎族支系DYS287、DYS19的多态性研究[J].遗传,2003,25(1):5-8. |
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| 作者姓名： | 李冬娜 应大君 区采莹 陈路 符生苗 谢协驹 陈峰 |
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| 作者单位： | 1.第三军医大学解剖学教研室，重庆 400038;2.海南医学院,海口 570102;3.海南省人民医院遗传室,海口 570311;4.哈尔滨医科大学医学遗传教研室,哈尔滨 150086 |
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| 基金项目： | 国家自然科学基金(39960033)资助 |
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| 摘 要： | 以中国海南岛（289例）本地黎（97例）、杞黎（96例）和侾黎（96例）人群为研究对象，采用PCR技术及变性聚丙烯酰胺凝胶电泳方法分析了Y染色体特异性微卫星DYS19的等位基因分布规律。本地黎群体检出4种等位基因，杞黎和侾黎群体均检出3种。在3个支系的等位基因中均以194bp的频率为最高，分别是0.711、0.855和0.667。杞黎与侾黎人群DYS19基因座的等位基因频率有极显著差异（P＜0.01）。我们还通过PCR方法调查了3个黎族支系的Alu序列插入基因座DYS287的多态性，结果显示：3个人群均未发生Alu序列插入。
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| 关 键 词： | 微卫星 Alu序列 多态性 Y染色体 黎族 |
| 文章编号： | 0253-9772(2003)01-0005-04 |
| 修稿时间： | 2002年3月18日 |
Polymorphism Analysis for DYS19 and DYS287 of Three Li Populations in Haina Island,China |
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| Dong-Na Li,Da-Jun Ying,Cai-Ying Ou,Lu Chen,Sheng-Miao Fu,Xie-Ju Xie,Feng Chen.Polymorphism Analysis for DYS19 and DYS287 of Three Li Populations in Haina Island,China[J].Hereditas,2003,25(1):5-8. |
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| Authors: | Dong-Na Li Da-Jun Ying Cai-Ying Ou Lu Chen Sheng-Miao Fu Xie-Ju Xie Feng Chen |
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| Institution: | 1.Department of Anatomy,The Third Military Medical University,Chongqing,400038 China;2.Hainan Medical College,Haikou,570102 China;3.People Hospital of Hainan Province,Haikou 570311 China;4.Department of Medical Biology,Harbin Medical University,Harbin,150086 China |
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| Abstract: | The distribution of Y-chromosome specific microsatellite DYS19, in 289 males of three ethnic populations in Hainan Island was studied with the method of PCR followed by denatured polyacrylamide gel electrophoresis and silver staining. The three ethnic populations are Bendi Li, Qi Li and Ha Li. The results showed that four alleles (190bp, 194bp, 198bp, 202bp designated as alleles B, C, D and E respectively), were observed in DYS19, the frequency ranged from 0.660-0.854, and was predominant in three Li subgroups. The pairwise comparison with Fisher's Exact test reveals that there exists significant difference (P<0.01) in DYS19 phenotype distribution between Qi Li and Ha Li populations. The polymorphism of the Y-chromosome specific Alu insert sequence DYS287 was investigated also in three Li populations. The results demonstrated that with 150 bp product in all Bendi-Li, Qi-li and Ha-li, YAP element is absent. The preliminary study provides reference populations for detecting male migration events and for reconstructing paternal history. |
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| Keywords: | ethnic Y-chromosome polymorphism microsatellite Alu sequence |
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