M.Smal is an N4-methylcytosine specific DNA-methylase. |
| |
| Authors: | S Klimasauskas D Steponaviciene Z Maneliene M Petrusyte V Butkus and A Janulaitis |
| |
| Institution: | Institute of Applied Enzymology, Vilnius, Lithuania, USSR. |
| |
| Abstract: | An enzymatic activity rendering DNA immune to the action of the Smal restriction endonuclease in the presence of S-adenosyl-L-methionine has been detected in Serratia marcescens Sb. This methylase, M.Smal, modifies the second cytosine residue of the substrate sequence CCCGGG yielding N4-methylcytosine. |
| |
| Keywords: | |
|
|
